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通过复制模式研究胸苷、氟脱氧尿苷、羟基脲和甲氨蝶呤在细胞周期G1/S期转换时的阻断作用比较。

Comparison of thymidine, fluorodeoxyuridine, hydroxyurea, and methotrexate blocking at the G1/S phase transition of the cell cycle, studied by replication patterns.

作者信息

Vogel W, Schempp W, Sigwarth I

出版信息

Hum Genet. 1978 Dec 18;45(2):193-8. doi: 10.1007/BF00286962.

Abstract

Cultures of human amniotic fluid cells and fibroblasts were temporarily blocked by the replication inhibitors thymidine (dT) surplus, fluorodeoxyuridine (FdU), hydroxyurea (HU), or methotrexate uridine (MU). The respective arresting point at G1-S transition and the homogeneity of the blocked cell population were determined by means of BrdU replication patterns. Most variation of patterns were found after HU. After MU, cells were arrested before the onset of replication, while with dT surplus of FdU an arresting point in early S seemed more likely.

摘要

人羊水细胞和成纤维细胞的培养物被复制抑制剂过量的胸苷(dT)、氟脱氧尿苷(FdU)、羟基脲(HU)或甲氨蝶呤尿苷(MU)暂时阻断。通过BrdU复制模式确定在G1-S转换处的各自停滞点以及被阻断细胞群体的同质性。在HU处理后发现模式的变化最多。在MU处理后,细胞在复制开始前被阻断,而在dT或FdU过量时,早期S期的停滞点似乎更有可能。

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