Karara A, Wei S, Spady D, Swift L, Capdevila J H, Falck J R
Department of Medicine (Nephrology), Vanderbilt University Medical School, Nashville, TN 37232.
Biochem Biophys Res Commun. 1992 Feb 14;182(3):1320-5. doi: 10.1016/0006-291x(92)91877-s.
Gas chromatographic/mass spectroscopic and chiral analysis showed the presence of enzymatically derived 8,9-, 11,12- and 14,15-EET in rat plasma (2.8:1:3.4 molar ratio, respectively; 10.2 +/- 0.4 ng total EET/ml plasma). Greater than 90% of the plasma EETs was esterified to the phospholipids of circulating lipoproteins. The lipoprotein fraction with the highest EET concentration was LDL (8.1 +/- 0.9 ng/mg of protein) followed by HDL and VLDL (3.5 +/- 0.1 and 1.9 +/- 0.3 ng/mg of protein, respectively). In light of the biological activities of the EETs, these results suggest a potential systemic function for the cytochrome P-450 epoxygenase.
气相色谱/质谱联用及手性分析表明，大鼠血浆中存在酶促衍生的8,9-、11,12-和14,15-环氧二十碳三烯酸（EET）（摩尔比分别为2.8:1:3.4；血浆中EET总量为10.2±0.4 ng/ml）。血浆中超过90%的EET与循环脂蛋白的磷脂酯化。EET浓度最高的脂蛋白组分是低密度脂蛋白（LDL）（8.1±0.9 ng/mg蛋白质），其次是高密度脂蛋白（HDL）和极低密度脂蛋白（VLDL）（分别为3.5±0.1和1.9±0.3 ng/mg蛋白质）。鉴于EET的生物学活性，这些结果提示细胞色素P-450环氧化酶可能具有潜在的全身性功能。
