用环氧乙烷在体外诱导人二倍体成纤维细胞产生6-硫鸟嘌呤抗性突变体。

Induction of 6-thioguanine-resistant mutants in human diploid fibroblasts in vitro with ethylene oxide.

作者信息

Kolman A, Bohusová T, Lambert B, Simons J W

机构信息

Department of Radiobiology, Stockholm University, Sweden.

出版信息

Environ Mol Mutagen. 1992;19(2):93-7. doi: 10.1002/em.2850190203.

DOI:10.1002/em.2850190203

PMID:1541259

Abstract

Human diploid fibroblasts (strain VH-10) were exposed to the direct-acting alkylating agent, ethylene oxide (EtO), in vitro, and the frequency of HPRT mutants was evaluated by selection in medium containing 6-thioguanine. A dose-dependent increase of the mutant frequency was found in the dose range of 2.5-10 mMh of EtO. The EtO-induced mutant frequency increased 5-19 times the background frequency at low or moderately toxic doses, which indicates that EtO is a strong mutagen in human fibroblasts in vitro. The mutagenic potency was 9.8 x 10(-6) per mMh.

摘要

将人二倍体成纤维细胞（VH - 10株）在体外暴露于直接作用的烷化剂环氧乙烷（EtO），通过在含有6 - 硫鸟嘌呤的培养基中进行筛选来评估次黄嘌呤 - 鸟嘌呤磷酸核糖转移酶（HPRT）突变体的频率。在2.5 - 10 mMh的EtO剂量范围内，发现突变频率呈剂量依赖性增加。在低或中等毒性剂量下，EtO诱导的突变频率比背景频率增加了5 - 19倍，这表明EtO在体外人成纤维细胞中是一种强诱变剂。诱变效力为每mMh 9.8×10⁻⁶。