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1
Mutations induced in the hypoxanthine phosphoribosyl transferase gene by three urban air pollutants: acetaldehyde, benzo[a]pyrene diolepoxide, and ethylene oxide.三种城市空气污染物（乙醛、苯并[a]芘二环氧物和环氧乙烷）诱导次黄嘌呤磷酸核糖转移酶基因发生的突变。
Environ Health Perspect. 1994 Oct;102 Suppl 4(Suppl 4):135-8. doi: 10.1289/ehp.94102s4135.
2
A mouse model for the study of in vivo mutational spectra: sequence specificity of ethylene oxide at the hprt locus.一种用于体内突变谱研究的小鼠模型：环氧乙烷在次黄嘌呤磷酸核糖转移酶基因座的序列特异性
Mutat Res. 1993 Jul;288(1):151-62. doi: 10.1016/0027-5107(93)90216-3.
3
Molecular analysis of ethylene oxide-induced mutations at the HPRT locus in human diploid fibroblasts.人二倍体成纤维细胞中环氧乙烷诱导的次黄嘌呤-鸟嘌呤磷酸核糖转移酶（HPRT）基因座突变的分子分析。
Mutat Res. 1993 Jun;287(2):283-92. doi: 10.1016/0027-5107(93)90021-7.
4
Mutations induced by benzo[a]pyrene diolepoxide at the hprt locus in human T-lymphocytes in vitro.
Mutat Res. 1990 Oct;245(2):75-82. doi: 10.1016/0165-7992(90)90003-3.
5
Strand specificity for mutations induced by (+)-anti BPDE in the hprt gene in human T-lymphocytes.
Mutat Res. 1992 Sep;269(1):129-40. doi: 10.1016/0027-5107(92)90168-2.
6
Nickel subsulfide is similar to potassium dichromate in protecting normal human fibroblasts from the mutagenic effects of benzo[a]pyrene diolepoxide.硫化亚镍在保护正常人成纤维细胞免受苯并[a]芘二环氧物的诱变作用方面与重铬酸钾相似。
Environ Mol Mutagen. 1999;33(3):211-8. doi: 10.1002/(sici)1098-2280(1999)33:3<211::aid-em5>3.0.co;2-7.
7
In vivo mutagenicity of ethylene oxide at the hprt locus in T-lymphocytes of B6C3F1 lacI transgenic mice following inhalation exposure.吸入暴露后B6C3F1 lacI转基因小鼠T淋巴细胞hprt基因座处环氧乙烷的体内诱变性。
Mutat Res. 1997 Aug 14;392(3):211-22. doi: 10.1016/s1383-5718(97)00062-4.
8
Acetaldehyde-induced mutation at the hprt locus in human lymphocytes in vitro.乙醛在体外诱导人淋巴细胞次黄嘌呤磷酸核糖转移酶基因座发生突变。
Environ Mol Mutagen. 1990;16(2):57-63. doi: 10.1002/em.2850160202.
9
Molecular analysis of styrene oxide-induced hprt mutation in human T-lymphocytes.环氧苯乙烷诱导人T淋巴细胞hprt基因突变的分子分析。
Mutagenesis. 1996 Nov;11(6):581-91. doi: 10.1093/mutage/11.6.581.
10
Hydrogen peroxide induced mutations at the HPRT locus in primary human T-lymphocytes.过氧化氢可诱导原代人T淋巴细胞中次黄嘌呤-鸟嘌呤磷酸核糖转移酶（HPRT）基因座发生突变。
Mutat Res. 2000 Aug 21;469(1):51-61. doi: 10.1016/s1383-5718(00)00058-9.

引用本文的文献

1
Genomic assay reveals tolerance of DNA damage by both translesion DNA synthesis and homology-dependent repair in mammalian cells.基因组分析揭示了哺乳动物细胞中转录跨越损伤 DNA 合成和同源依赖性修复对 DNA 损伤的耐受性。
Proc Natl Acad Sci U S A. 2013 Apr 16;110(16):E1462-9. doi: 10.1073/pnas.1216894110. Epub 2013 Mar 25.
2
Future research needs associated with the assessment of potential human health risks from exposure to toxic ambient air pollutants.与评估接触有毒环境空气污染物对人类健康的潜在风险相关的未来研究需求。
Environ Health Perspect. 1994 Oct;102 Suppl 4(Suppl 4):193-210. doi: 10.1289/ehp.102-1566924.

本文引用的文献

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Molecular spectrum of background mutation at the hprt locus in human T-lymphocytes.人T淋巴细胞中次黄嘌呤磷酸核糖转移酶（hprt）基因座背景突变的分子谱。
Mutagenesis. 1993 Jan;8(1):43-9. doi: 10.1093/mutage/8.1.43.
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Molecular analysis of ethylene oxide-induced mutations at the HPRT locus in human diploid fibroblasts.人二倍体成纤维细胞中环氧乙烷诱导的次黄嘌呤-鸟嘌呤磷酸核糖转移酶（HPRT）基因座突变的分子分析。
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T-cell cloning to detect the mutant 6-thioguanine-resistant lymphocytes present in human peripheral blood.通过T细胞克隆检测人类外周血中存在的对6-硫鸟嘌呤耐药的突变淋巴细胞。
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Mutation in mammalian cells by stereoisomers of anti-benzo[a] pyrene-diolepoxide in relation to the extent and nature of the DNA reaction products.
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Molecular evidence for new mutation at the hprt locus in Lesch-Nyhan patients.莱施-奈恩综合征患者次黄嘌呤磷酸核糖基转移酶（HPRT）基因座新突变的分子证据。
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Somatic gene mutations in vivo as indicated by the 6-thioguanine-resistant T-lymphocytes in human blood.人体血液中抗6-硫鸟嘌呤T淋巴细胞所表明的体内体细胞基因突变。
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三种城市空气污染物（乙醛、苯并[a]芘二环氧物和环氧乙烷）诱导次黄嘌呤磷酸核糖转移酶基因发生的突变。

Mutations induced in the hypoxanthine phosphoribosyl transferase gene by three urban air pollutants: acetaldehyde, benzo[a]pyrene diolepoxide, and ethylene oxide.

作者信息

Lambert B, Andersson B, Bastlova T, Hou S M, Hellgren D, Kolman A

机构信息

Environmental Medicine Unit, Karolinska Institutet, Huddinge, Sweden.

出版信息

Environ Health Perspect. 1994 Oct;102 Suppl 4(Suppl 4):135-8. doi: 10.1289/ehp.94102s4135.

DOI:10.1289/ehp.94102s4135
PMID:7821287
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1566944/
Abstract

Provisional mutational spectra at the hypoxanthine phosphoribosyl transferase (HPRT) locus in vitro have been worked out for acetaldehyde (AA) and benzo[a]pyrene diolepoxide (BPDE) in human (T)-lymphocytes and for ethylene oxide (EtO) in human diploid fibroblasts using Southern blotting and polymerase chain reaction (PCR)-based DNA sequencing techniques. The results indicate that large genomic deletions are the predominating hprt mutations caused by AA and EO, whereas BPDE induces point mutations that are mainly GC > TA transversions. The mutational spectra induced by the three agents are clearly different from the background spectrum in human T-cells. Thus, the hprt locus is a useful target for the study of chemical-specific mutational events that may help identify causes of background mutation in human cells in vivo.

摘要

利用Southern印迹法和基于聚合酶链反应（PCR）的DNA测序技术，已得出人（T）淋巴细胞中乙醛（AA）和苯并[a]芘二环氧物（BPDE）以及人二倍体成纤维细胞中环氧乙烷（EtO）在次黄嘌呤磷酸核糖转移酶（HPRT）基因座的临时突变谱。结果表明，大片段基因组缺失是由AA和环氧乙烷引起的主要hprt突变，而BPDE诱导的点突变主要是GC>TA颠换。这三种试剂诱导的突变谱明显不同于人T细胞中的背景谱。因此，hprt基因座是研究化学特异性突变事件的有用靶点，这可能有助于确定体内人类细胞背景突变的原因。