Müller F W, Igloi G L, Beck C F
Institut für Biologie III, Albert-Ludwigs-Universität, Freiburg, F.R.G.
Gene. 1992 Feb 15;111(2):165-73. doi: 10.1016/0378-1119(92)90684-h.
The structure of a gene encoding a 70-kDa heat-shock protein (HSP70) from the unicellular alga, Chlamydomonas reinhardtii, is described. This gene shows a remarkable expression pattern, because it is inducible by light as well as by elevated temperature [von Gromoff et al., Mol. Cell. Biol. 9 (1989) 3911-3918]. As a first step in the investigation of trans-acting factors involved in environmentally controlled expression of this hsp70 gene, the nucleotide sequence of the entire gene, including its 5'- and 3'-flanking regions was determined. Although the deduced amino acid sequence exhibits a high degree of conservation to the HSP70 from higher plants, the C. reinhardtii gene has a unique structure among the members of the hsp70 gene family. While most hsp70 genes have only one or no intron, the coding region of the C. reinhardtii gene is interrupted by six introns. Besides putative TATA and CCAAT boxes, two heat-shock elements (HSE) were found in the promoter region, and a third HSE motif was located within the fourth intron. A computer search for regulatory cis-acting elements revealed a noted similarity of a 5'-upstream sequence motif to the G-box motif conserved in higher plants. A polyadenylation recognition sequence canonical for nuclear genes of C. reinhardtii is located downstream from the coding sequence.
本文描述了单细胞藻类莱茵衣藻中编码70 kDa热休克蛋白（HSP70）的基因结构。该基因呈现出显著的表达模式，因为它可被光以及高温诱导[冯·格罗莫夫等人，《分子细胞生物学》9 (1989) 3911 - 3918]。作为研究参与该hsp70基因环境调控表达的反式作用因子的第一步，测定了整个基因的核苷酸序列，包括其5'和3'侧翼区域。尽管推导的氨基酸序列与高等植物的HSP70具有高度保守性，但莱茵衣藻基因在hsp70基因家族成员中具有独特的结构。大多数hsp70基因只有一个内含子或没有内含子，而莱茵衣藻基因的编码区被六个内含子打断。除了推定的TATA盒和CCAAT盒外，在启动子区域发现了两个热休克元件（HSE），第三个HSE基序位于第四个内含子内。对调控顺式作用元件的计算机搜索显示，5'上游序列基序与高等植物中保守的G盒基序有显著相似性。莱茵衣藻核基因典型的聚腺苷酸化识别序列位于编码序列下游。
