La Rosa M, Sconzo G, Giudice G, Roccheri M C, Di Carlo M
Dipartimento di Biologia Cellulare e dello Sviluppo, Università di Palermo, Italy.
Gene. 1990 Dec 15;96(2):295-300. doi: 10.1016/0378-1119(90)90267-u.
We report the nucleotide sequence of a 4470-bp fragment derived from a sea urchin genomic clone containing part of a heat-shock protein 70 (Hsp70)-encoding gene. This fragment, named hsp70 gene II, contains 1271 bp of the flanking region and 3299 bp of structural gene sequence interrupted by five introns and encoding the N-terminal 371 amino acids (aa) of the protein. The 5' flanking region contains a putative TATA element, two CCAAT boxes, four heat-shock consensus sequence elements (hse) and one consensus sequence for binding of Sp1. Remarkable homologies were observed for deduced aa sequence and intron-exon organization between hsp70 gene II and rat hsc73 gene.
我们报道了来自海胆基因组克隆的一个4470碱基对片段的核苷酸序列，该克隆包含部分热休克蛋白70（Hsp70）编码基因。这个片段名为hsp70基因II，包含1271碱基对的侧翼区域和3299碱基对的结构基因序列，该序列被五个内含子中断，并编码该蛋白质的N端371个氨基酸。5'侧翼区域包含一个假定的TATA元件、两个CCAAT框、四个热休克共有序列元件（hse）和一个Sp1结合的共有序列。在hsp70基因II和大鼠hsc73基因之间，推导的氨基酸序列和内含子-外显子组织存在显著同源性。
