Thal L J, Gilbertson E, Armstrong D M, Gage F H
Department of Neurosciences, University of California, San Diego, La Jolla 92093.
Neurobiol Aging. 1992 Jan-Feb;13(1):67-72. doi: 10.1016/0197-4580(92)90011-l.
We measured choline acetyltransferase (ChAT) and acetylcholinesterase (AChE) activities in the rat to determine the time course of development, maturity, and senescence of ChAT activity. Tissue was obtained from Sprague-Dawley rats ranging in age from embryonic day 14 through 23 months. Seven regions were examined, including the magnocellular preoptic/substantia innominata region, frontal cortex, medial septal region, hippocampus, diagnoal band, and medial and lateral striatum. ChAT and AChE activities were first detected as early as E18 in the medial septum, diagonal band and magnocellular preoptic area, all regions of cholinergic cell bodies. Enzyme activity subsequently developed in terminal fields of these cholinergic perikarya (hippocampus and frontal cortex) as well as in the striatum. For all regions, enzyme activity rose during the first four postnatal weeks. This increase in enzyme activity was transient and, in most instances, decreases were observed between postnatal days 30 and 60. Most dramatic were the decreases in enzyme activity in the magnocellular preoptic/substantia innominata and diagonal band regions. Age-related declines also occurred in the frontal cortex, hippocampus, magnocellular preoptic/substantia innominata region, and the striatum. Cholinergic systems undergo dynamic changes especially during development and adulthood.
我们测量了大鼠体内的胆碱乙酰转移酶（ChAT）和乙酰胆碱酯酶（AChE）活性，以确定ChAT活性的发育、成熟和衰老的时间进程。组织取自年龄范围从胚胎第14天到23个月的Sprague-Dawley大鼠。检测了七个区域，包括大细胞视前区/无名质区、额叶皮质、内侧隔区、海马体、斜角带以及内侧和外侧纹状体。ChAT和AChE活性最早在胚胎第18天在内侧隔区、斜角带和大细胞视前区被检测到，这些都是胆碱能细胞体所在区域。酶活性随后在这些胆碱能神经元胞体的终末区域（海马体和额叶皮质）以及纹状体中发展。对于所有区域，酶活性在出生后的前四周内上升。这种酶活性的增加是短暂的，在大多数情况下，在出生后第30天至60天之间观察到活性下降。最显著的是大细胞视前区/无名质区和斜角带区域的酶活性下降。额叶皮质、海马体、大细胞视前区/无名质区和纹状体也出现了与年龄相关的下降。胆碱能系统会经历动态变化，尤其是在发育和成年期。
