Woolf N J, Butcher L L
Brain Res Bull. 1985 Jan;14(1):63-83. doi: 10.1016/0361-9230(85)90178-9.
The cholinergic innervation of the interpeduncular nucleus was investigated by use of fluorescent tracer histology in combination with choline-O-acetyltransferase (ChAT) immunohistochemistry and acetylcholinesterase (AChE) pharmacohistochemistry. Following propidium iodide or Evans Blue infusion into the interpeduncular nucleus, brains were processed for co-localization of transported fluorescent label and ChAT and AChE. Control infusions of tracers were made into the ventral tegmental area. In order to delimit the course of putative cholinergic afferents to the interpeduncular nucleus from extra-habenular sources, knife cuts surrounding the habenular nuclei were performed. Somata containing propidium iodide that were highly immunoreactive for ChAT were found primarily in the vertical and horizontal limbs of the diagonal band, the magnocellular preoptic area, and the dorsolateral tegmental nucleus, also referred to as the laterodorsal tegmental nucleus. A few such co-labeled somata were also detected in the medial septal nucleus, substantia innominata, nucleus basalis, and pedunculopontine tegmental nucleus. A good correlation was observed between intensely-staining, AChE-containing and ChAT-positive neurons projecting to the interpeduncular nucleus from the aforementioned structures. Although the medial habenula contained numerous cells demonstrating transported label following interpeduncular infusion of fluorescent tracers, the ChAT-positivity associated with somata in that nucleus was weak compared to ChAT-like immunoreactivity in known cholinergic neurons in the basal forebrain and brainstem. Knife cuts that separated the habenular nuclei from the stria medullaris and neural regions lateral and posterior to those nuclei while leaving the fasciculus retroflexus intact resulted in a reduction of ChAT-like immunoreactivity in the medial habenular nucleus, fasciculus retroflexus, and interpeduncular nucleus. These data suggest (1) that the cholinergic innervation of the interpeduncular nucleus derives primarily from ChAT-positive cells in the basal forebrain and dorsolateral tegmental nucleus and (2) that putative cholinergic fibers having their origin in the medial habenula, if they exist, constitute a minor portion of the cholinergic input to the interpeduncular nucleus.
运用荧光示踪组织学技术，并结合胆碱 - O - 乙酰转移酶（ChAT）免疫组织化学和乙酰胆碱酯酶（AChE）药物组织化学方法，对脚间核的胆碱能神经支配进行了研究。在将碘化丙啶或伊文思蓝注入脚间核后，对大脑进行处理，以观察转运荧光标记与ChAT和AChE的共定位情况。将示踪剂对照注入腹侧被盖区。为了界定来自缰核外来源的假定胆碱能传入纤维至脚间核的行程，对围绕缰核的区域进行了刀切损伤。发现含有碘化丙啶且对ChAT具有高度免疫反应性的胞体主要位于斜角带垂直部和水平部、视前大细胞区以及背外侧被盖核（也称为外侧背被盖核）。在内侧隔核、无名质、基底核和脚桥被盖核中也检测到了一些这样的共标记胞体。观察到从上述结构投射至脚间核的、强烈染色的含AChE且ChAT阳性的神经元之间存在良好的相关性。尽管在脚间核注入荧光示踪剂后，内侧缰核含有大量显示有转运标记的细胞，但与基底前脑和脑干中已知胆碱能神经元的ChAT样免疫反应性相比，该核中胞体的ChAT阳性较弱。将缰核与髓纹以及这些核外侧和后方的神经区域分离，同时保留后屈束完整的刀切损伤，导致内侧缰核、后屈束和脚间核中ChAT样免疫反应性降低。这些数据表明：（1）脚间核的胆碱能神经支配主要源自基底前脑和背外侧被盖核中的ChAT阳性细胞；（2）如果存在，起源于内侧缰核的假定胆碱能纤维仅构成脚间核胆碱能输入的一小部分。
