使用第11届国际组织相容性研讨会试剂对意大利乳糜泻患者进行寡核苷酸分型。

Oligotyping of Italian celiac patients with the 11th International Histocompatibility Workshop reagents.

作者信息

Ferrante P, Petronzelli F, Mariani P, Bonamico M, Mazzilli M C

机构信息

Department of Experimental Medicine, University La Sapienza, Rome, Italy.

出版信息

Tissue Antigens. 1992 Jan;39(1):38-9. doi: 10.1111/j.1399-0039.1992.tb02155.x.

DOI:10.1111/j.1399-0039.1992.tb02155.x

PMID:1542877

Abstract

Oligotyping for HLA-DRB1, DQA1 and DQB1 specificities has been performed on PCR-amplified DNA from 55 Italian celiac children, living in Rome, and 50 blood donors. 52.6% of CD patients were DR3;DQA10501;DQB10201-positive versus 14% of controls (RR = 6.85) and 34.5% were DR5,7;DQA1DQB10201-positive versus 2% of controls (RR = 25.86). 7 patients (12.7%) were negative for the DQA10501/B10201 dimer: 3 of them were DR4 (5.4%) and the others typed as DR1,5; 1,7; 5,7 and w6,7. No patient was negative for both DQA10501 and DQB10201 alleles.

摘要

已对来自55名居住在罗马的意大利乳糜泻儿童和50名献血者的PCR扩增DNA进行了HLA - DRB1、DQA1和DQB1特异性寡核苷酸分型。52.6%的乳糜泻患者为DR3;DQA10501;DQB10201阳性，而对照组为14%（相对风险率RR = 6.85）；34.5%为DR5,7;DQA1DQB10201阳性，而对照组为2%（RR = 25.86）。7名患者（12.7%）DQA10501/B10201二聚体呈阴性：其中3名是DR4（5.4%），其他分型为DR1,5；1,7；5,7和w6,7。没有患者DQA10501和DQB10201等位基因均为阴性。