Gebuhrer L, Lambert J, Labonne M P, Mollet I, Freidel A C, Betuel H
Histocompatibility Laboratory, Blood Transfusion Center, Lyon, France.
Tissue Antigens. 1992 Jan;39(1):42-7. doi: 10.1111/j.1399-0039.1992.tb02157.x.
Oligotyping has revealed a considerable polymorphism of HLA DRw13; so far, 5 alleles coded by DRB1 have been identified. An even greater number of haplotypes are apparent when considering the association of DRw13 with alleles coded by DRB3 and DQB1. Serology can now define some of these variants which had formerly escaped detection. We have tested by serology and by oligoprobes 66 genotyped Caucasoid cells comprising the known DR and DQ alleles. Among the 28 DRw6 cells, 10 different haplotype patterns were selected, of which 8 concern DRw13 and 2 DRw14. Four variants of DRw13 were represented: DRB1 *1301, *1302, *1303 and *1305 associated with usual and with uncommon alleles belonging to DRB3 and to DQB1. We have centered our analysis on 19 sera of the XIth Workshop defining DRw6. Two sera (639, 826) were noteworthy since they reacted with certain subtypes of DRw13 and with DRB1 *1102, *1103 only, the other DR specificities remaining negative.
寡核苷酸分型揭示了HLA DRw13存在相当大的多态性；到目前为止，已鉴定出由DRB1编码的5个等位基因。当考虑DRw13与由DRB3和DQB1编码的等位基因的关联时，单倍型的数量甚至更多。血清学现在可以鉴定出一些以前未被检测到的变体。我们通过血清学和寡核苷酸探针检测了66个已知DR和DQ等位基因的白种人细胞系。在28个DRw6细胞系中，选择了10种不同的单倍型模式，其中8种与DRw13有关，2种与DRw14有关。DRw13有4种变体：DRB1 *1301、*1302、1303和1305，它们与属于DRB3和DQB1的常见和罕见等位基因相关。我们将分析集中在第十一届研讨会定义DRw6的19份血清上。两份血清（639、826）值得注意，因为它们仅与DRw13的某些亚型以及DRB1 *1102、*1103发生反应，其他DR特异性均为阴性。
