通过血清学Dw分型和限制性片段长度多态性揭示的DRw13 DQw7单倍型之间的多样性。

Diversity among DRw13 DQw7 haplotypes as revealed by serology Dw typing and restriction fragment length polymorphism.

作者信息

Gebuhrer L, Freidel A C, Lambert J, Labonne M P, Farre A, Betuel H

机构信息

Histocompatibility Laboratory, Blood Transfusion Centre, Lyon, France.

出版信息

Hum Immunol. 1991 Feb;30(2):85-90. doi: 10.1016/0198-8859(91)90075-k.

DOI:10.1016/0198-8859(91)90075-k

PMID:1673674

Abstract

HLA-DRw13 is in linkage disequilibrium with DQw6; an unusual association, DRw13 DQw7, is found in 2% of our Caucasoid population. Investigation of genotyped individuals and of families by two allosera and by Dw typing revealed two subtypes: one recognized by homozygous typing cell HAG and by two allosera, the other subtype remained unreactive with both reagents. Analysis of DNA fragments with DNA probes indicated that the HAG-negative subset had DNA fragments in common with DRw6 while the HAG-positive subset shared DNA fragments with DR5. However, all DRw13 DQw7 cells are Dw24 as seen by hybridization with DRB probe.

摘要

HLA - DRw13与DQw6处于连锁不平衡状态；在我们2%的白种人群中发现了一种不寻常的关联，即DRw13 DQw7。通过两种同种抗血清和Dw分型对基因分型个体和家庭进行调查，发现了两个亚型：一个可被纯合分型细胞HAG和两种同种抗血清识别，另一个亚型与两种试剂均无反应。用DNA探针分析DNA片段表明，HAG阴性亚群具有与DRw6相同的DNA片段，而HAG阳性亚群与DR5共享DNA片段。然而，通过与DRB探针杂交可见，所有DRw13 DQw7细胞均为Dw24。