Dubes G R, Wegrzyn R J
Acta Virol. 1977 Jan;21(1):15-24.
Kinetics of sensitization of chimpanzee cell sheets to transfection by poliovirus RNA was determined for 5 basic polypeptides. With basic olypeptide hydrobromide at 100 microng/ml, initial sensitization rate was faster for poly-L-ornithine of average molecular weight (AMW) 15500 than of AMW 105000, and much faster for poly-L-lysine of AMW 1700 than of AMW 140000. Desensitization phases were observed with the 2 shorter polypeptides. Sensitization was much faster and sensitivity maxima were considerably higher for the polyornithines than for the polylysines. Poly-D-lysine of AMW 160000 sensitized cells slightly faster than poly-L-lysine of AMW 140000, but gave about the same sensitivity maximum. Analysis of the slow cell sensitization by poly-L-lysine of AMW 140000 revealed 2 steps: a fast step 1 (attachment) and a slow step 2 (processing).
测定了5种碱性多肽使黑猩猩细胞片对脊髓灰质炎病毒RNA转染的致敏动力学。对于浓度为100微克/毫升的碱性多肽氢溴酸盐，平均分子量（AMW）为15500的聚-L-鸟氨酸的初始致敏速率比AMW为105000的更快，而AMW为1700的聚-L-赖氨酸的初始致敏速率比AMW为140000的快得多。观察到两种较短多肽存在脱敏阶段。聚鸟氨酸的致敏速度更快，最大敏感度比聚赖氨酸高得多。AMW为160000的聚-D-赖氨酸使细胞致敏的速度略快于AMW为140000的聚-L-赖氨酸，但最大敏感度大致相同。对AMW为140000的聚-L-赖氨酸引起的缓慢细胞致敏分析显示有两个步骤：快速的步骤1（附着）和缓慢的步骤2（加工）。
