Bakker L J, Nottet H S, de Vos N M, de Graaf L, Van Strijp J A, Visser M R, Verhoef J
Department of Clinical Microbiology, University Hospital Utrecht, The Netherlands.
AIDS. 1992 Jan;6(1):35-41. doi: 10.1097/00002030-199201000-00004.
To characterize antibody- and complement-mediated binding and uptake of HIV-1 by human monocytes.
The first step in the infection of the monocyte by HIV-1 is binding of the virus to the susceptible cell. Procedures were designed to assess the influence of anti-HIV-1 antibodies and complement on this binding, and to study the process of internalization following binding.
Human monocytes were incubated with fluorescein-labelled purified HTLV-IIIB virions and human sera with high-titre anti-HIV-1 antibodies and/or complement. Binding and uptake of virus by the monocytes was measured as fluorescence per cell by flow cytometry.
Binding of purified HIV-1 to monocytes was increased by complement and, to a lesser extent, by anti-HIV-1 antibodies. Uptake of HIV-1 bound to the monocyte appeared to be mediated by antibodies and was increased further by the presence of complement. Complement alone, however, resulted in the uptake of only a small part of the bound virus.
Complement significantly increases the binding of HIV-1 to human monocytes, and a combination of antibodies and complement efficiently mediates uptake of HIV-1 by monocytes.
表征人单核细胞对HIV-1的抗体和补体介导的结合及摄取。
HIV-1感染单核细胞的第一步是病毒与易感细胞的结合。设计了相关程序来评估抗HIV-1抗体和补体对这种结合的影响，并研究结合后的内化过程。
将人单核细胞与荧光素标记的纯化HTLV-IIIB病毒颗粒以及含有高滴度抗HIV-1抗体和/或补体的人血清一起孵育。通过流式细胞术测量单核细胞对病毒的结合和摄取，以每个细胞的荧光强度表示。
补体增加了纯化的HIV-1与单核细胞的结合，抗HIV-1抗体在较小程度上也有此作用。与单核细胞结合的HIV-1的摄取似乎由抗体介导，补体的存在进一步增加了摄取。然而，单独的补体仅导致结合病毒的一小部分被摄取。
补体显著增加HIV-1与人单核细胞的结合，抗体和补体的组合有效地介导单核细胞对HIV-1的摄取。
