Kato H, Yamamura Y, Inoue M, Tanigawa M, Kimura S, Sugino S, Kondo M
First Department of Internal Medicine, Kyoto Prefectural University of Medicine, Japan.
Biotherapy. 1992;4(1):69-73. doi: 10.1007/BF02171712.
Peritoneal polymorphonuclear leukocytes (PMNs) were collected from the peritoneal cavity of C3H/He mice 6 hrs after intraperitoneal (i.p.) injection of 2.5 mg/head of PSK, 1 KE (100 micrograms)/head of OK-432 or 200 micrograms/head of Nocardia rubra cell wall skeleton (N-CWS). Without in vitro stimulation, these PMNs did not show cytotoxicity to syngeneic MM46 mammary carcinoma cells in 51Cr release assay. Cytotoxicity of these PMNs was augmented by the addition of 25 micrograms/ml of N-CWS but not of PSK or OK-432 to cultures for the assay at the beginning of the culture. H2O2 production of PSK-induced PMNs was increased by the in vitro addition of 25 micrograms/ml of N-CWS but not of PSK. These results suggest that PSK as well as OK-432 and N-CWS can induce PMNs capable of responding further to N-CWS as the second stimulant.
在腹腔注射2.5毫克/只PSK、1 KE（100微克）/只OK-432或200微克/只红色诺卡氏菌细胞壁骨架（N-CWS）6小时后，从C3H/He小鼠的腹腔中收集腹膜多形核白细胞（PMN）。在无体外刺激的情况下，这些PMN在51Cr释放试验中对同基因MM46乳腺癌细胞未表现出细胞毒性。在培养开始时，向用于试验的培养物中添加25微克/毫升的N-CWS可增强这些PMN的细胞毒性，但添加PSK或OK-432则不能。体外添加25微克/毫升的N-CWS可增加PSK诱导的PMN产生H2O2，但添加PSK则不能。这些结果表明，PSK以及OK-432和N-CWS均可诱导PMN，使其能够作为第二刺激物对N-CWS产生进一步反应。
