Lagrost L
Graduate School of Health and Medical Sciences, University of Wollongong, NSW, Australia.
Biochim Biophys Acta. 1992 Mar 4;1124(2):159-62. doi: 10.1016/0005-2760(92)90092-a.
In a previous publication (Lagrost, L. and Barter, P.J. (1991) Biochim. Biophys. Acta 1085, 209-216), saturated and cis unsaturated non-esterified fatty acids have been shown to modulate the rate at which cholesteryl esters are transferred from high-density lipoproteins (HDL) to low-density lipoproteins (LDL) in the presence of the human cholesteryl ester transfer protein (CETP). In the present report, the effects of cis (oleic acid) and trans (elaidic acid) monounsaturated isomers on the CETP-mediated transfer of cholesteryl esters between HDL and LDL were compared. Mixtures of human LDL and HDL3, containing or not radiolabelled cholesteryl esters, were incubated at 37 degrees C with CETP in the presence or in the absence of either stearic (18:0), oleic (18:1 cis) or elaidic (18:1 trans) acids. It was observed that oleic acid and elaidic acid had different effects on the CETP-mediated redistribution of radiolabelled cholesteryl esters as well as on the net mass transfer of cholesterol from HDL3 to LDL. In particular, at high non-esterified fatty acid/lipoprotein ratio, the transfer of cholesteryl esters was significantly inhibited by the cis isomer and increased by the trans isomer.
在之前的一篇文献中（Lagrost, L. 和 Barter, P.J. (1991)《生物化学与生物物理学报》1085, 209 - 216），已表明饱和及顺式不饱和非酯化脂肪酸在人胆固醇酯转运蛋白（CETP）存在的情况下，可调节胆固醇酯从高密度脂蛋白（HDL）向低密度脂蛋白（LDL）转移的速率。在本报告中，比较了顺式（油酸）和反式（反油酸）单不饱和异构体对CETP介导的HDL与LDL之间胆固醇酯转移的影响。含有或不含有放射性标记胆固醇酯的人LDL和HDL3混合物，在有或无硬脂酸（18:0）、油酸（18:1顺式）或反油酸（18:1反式）存在的情况下，于37℃与CETP一起孵育。观察到油酸和反油酸对CETP介导的放射性标记胆固醇酯的重新分布以及胆固醇从HDL3到LDL的净质量转移有不同影响。特别是，在高非酯化脂肪酸/脂蛋白比率下，顺式异构体显著抑制胆固醇酯的转移，而反式异构体则使其增加。
