Lagrost L, Barter P J
Baker Medical Research Institute, Melbourne, Victoria, Australia.
Biochim Biophys Acta. 1991 Mar 12;1082(2):204-10. doi: 10.1016/0005-2760(91)90195-n.
The effects of various non-esterified fatty acids on the CETP-mediated particle size redistribution of HDL were studied by incubating HDL3 and CETP for 24 h at 37 degrees C in the absence or in the presence of either saturated, monounsaturated or polyunsaturated non-esterified fatty acids. In the absence of non-esterified fatty acids, CETP induced a redistribution of the initial population of HDL3 (Stokes' radius 4.3 nm) by promoting the appearance of one larger (Stokes' radius 4.8 nm) and two smaller (Stokes' radii 3.9 and 3.7 nm) HDL subpopulations. Whereas the non-esterified fatty acids alone did not modify the HDL3 distribution profile, they were able to alter markedly the capacity of CETP to induce the particle size redistribution of HDL. All the saturated fatty acids with at least 10 carbons were able to increase the formation of the very small sized particles (Stokes' radius 3.7 nm) in a concentration dependent manner, the medium chain fatty acids (12 and 14 carbons) being the best activators. The potential effect of non-esterified fatty acids was also influenced by the presence of double bonds in their monomeric carbon chain. While at low concentrations of non-esterified fatty acids (0.1 mmol/l) the enhancement of the formation of very small HDL particles appeared to be greater with oleic and linoleic acids than with stearic acid, at higher concentrations (0.4 mmol/l), oleic, linoleic and arachidonic acids decreased the formation of the 3.7 nm radius particles. The inhibition of the process at high concentrations of unsaturated fatty acids was linked to the degree of unsaturation of their carbon chain, arachidonic acid being the strongest inhibitor. The present study has demonstrated that non-esterified fatty acids can modulate the particle size redistribution of HDL3 mediated by the cholesteryl ester transfer protein even in the absence of any other lipoprotein classes. The effect of non-esterified fatty acid is dependent on both the length and the degree of unsaturation of their monomeric carbon chain.
通过在37℃下将HDL3和胆固醇酯转运蛋白（CETP）在不存在或存在饱和、单不饱和或多不饱和非酯化脂肪酸的情况下孵育24小时，研究了各种非酯化脂肪酸对CETP介导的HDL粒径再分布的影响。在不存在非酯化脂肪酸的情况下，CETP通过促进出现一个较大的（斯托克斯半径4.8nm）和两个较小的（斯托克斯半径3.9和3.7nm）HDL亚群，诱导了初始HDL3群体（斯托克斯半径4.3nm）的再分布。虽然单独的非酯化脂肪酸不会改变HDL3的分布谱，但它们能够显著改变CETP诱导HDL粒径再分布的能力。所有至少含有10个碳的饱和脂肪酸都能够以浓度依赖的方式增加非常小尺寸颗粒（斯托克斯半径3.7nm）的形成，中链脂肪酸（12和14个碳）是最佳激活剂。非酯化脂肪酸的潜在作用也受到其单体碳链中双键存在的影响。在低浓度的非酯化脂肪酸（0.1mmol/L）下，油酸和亚油酸比硬脂酸更能促进非常小的HDL颗粒的形成，而在较高浓度（0.4mmol/L）下，油酸、亚油酸和花生四烯酸会减少3.7nm半径颗粒的形成。高浓度不饱和脂肪酸对该过程的抑制作用与其碳链的不饱和程度有关，花生四烯酸是最强的抑制剂。本研究表明，即使在不存在任何其他脂蛋白类别的情况下，非酯化脂肪酸也可以调节胆固醇酯转运蛋白介导的HDL3粒径再分布。非酯化脂肪酸的作用取决于其单体碳链的长度和不饱和程度。
