大鼠肝脏线粒体δ3,δ2-烯酰辅酶A异构酶的cDNA克隆
cDNA cloning of mitochondrial delta 3, delta 2-enoyl-CoA isomerase of rat liver.
作者信息
Tomioka Y, Hirose A, Moritani H, Hishinuma T, Hashimoto T, Mizugaki M
机构信息
Department of Pharmaceutical Sciences, Tohoku University Hospital, Miyagi, Japan.
出版信息
Biochim Biophys Acta. 1992 Feb 28;1130(1):109-12. doi: 10.1016/0167-4781(92)90473-d.
A 1.08 kbp cDNA encoding rat liver mitochondrial delta 3, delta 2-enoyl-CoA isomerase (ECI) of 298 amino acid residues (Mr 32,895) was isolated from rat liver lambda gt11, lambda gt10 cDNA libraries by the combination of an immunochemical method with a rabbit-antibody against rat liver ECI and a plaque hybridization method. The deduced amino acid sequence from the cDNA indicates that ECI is synthesized with an amino-terminal extrasequence of 35 amino acid residues and processed to the mature enzyme (Mr 29,256).
通过将免疫化学方法（使用抗大鼠肝脏Δ3,Δ2-烯酰辅酶A异构酶（ECI）的兔抗体）与噬菌斑杂交方法相结合，从大鼠肝脏λgt11、λgt10 cDNA文库中分离出一个1.08 kbp的cDNA，其编码由298个氨基酸残基组成（Mr 32,895）的大鼠肝脏线粒体Δ3,Δ2-烯酰辅酶A异构酶（ECI）。从该cDNA推导的氨基酸序列表明，ECI合成时带有一个35个氨基酸残基的氨基末端额外序列，并加工成成熟酶（Mr 29,256）。
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