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Δ3,Δ2-烯酰辅酶A异构酶是一种与人类谷胱甘肽S-转移酶从S-己基谷胱甘肽亲和基质中共纯化的蛋白质。

Delta 3, delta 2-enoyl-CoA isomerase is the protein that copurifies with human glutathione S-transferases from S-hexylglutathione affinity matrices.

作者信息

Takahashi Y, Hirata Y, Burstein Y, Listowsky I

机构信息

Department of Biochemistry, Albert Einstein College of Medicine, Bronx, NY 10461.

出版信息

Biochem J. 1994 Dec 15;304 ( Pt 3)(Pt 3):849-52. doi: 10.1042/bj3040849.

DOI:10.1042/bj3040849
PMID:7818490
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1137411/
Abstract

An unidentified 30 kDa protein frequently copurifies with human glutathione S-transferases from S-hexyl-glutathione affinity matrices. Application of two-step sequential affinity chromatographic methods yielded a homogeneous preparation of that protein from human liver specimens. The protein was digested with Achromobacter protease I, and sequences of peptides resolved by h.p.l.c. showed a high degree of identity with those of rat mitochondrial delta 3, delta 2-enoyl-CoA isomerase. The human protein also exhibited catalytic activity with delta 3-cis-octenyl CoA as a substrate. Thus it is identified as liver delta 3, delta 2-enoyl-CoA isomerase.

摘要

一种未知的30 kDa蛋白质经常与人谷胱甘肽S-转移酶从S-己基谷胱甘肽亲和基质中共同纯化出来。采用两步连续亲和色谱法从人肝脏标本中获得了该蛋白质的纯品。用无色杆菌蛋白酶I对该蛋白质进行消化，通过高效液相色谱法分离得到的肽段序列与大鼠线粒体δ3,δ2-烯酰辅酶A异构酶的序列高度同源。该人类蛋白质也以δ3-顺式辛烯酰辅酶A为底物表现出催化活性。因此，它被鉴定为肝脏δ3,δ2-烯酰辅酶A异构酶。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c250/1137411/86b605d52e68/biochemj00073-0191-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c250/1137411/f66143499a1a/biochemj00073-0190-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c250/1137411/86b605d52e68/biochemj00073-0191-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c250/1137411/f66143499a1a/biochemj00073-0190-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c250/1137411/86b605d52e68/biochemj00073-0191-a.jpg

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本文引用的文献

1
Beta-oxidation of unsaturated fatty acids in humans. Isoforms of delta 3, delta 2-enoyl-CoA isomerase.人类中不饱和脂肪酸的β-氧化。δ3、δ2-烯酰辅酶A异构酶的同工型。
FEBS Lett. 1993 May 17;322(3):299-303. doi: 10.1016/0014-5793(93)81590-v.
2
A basis for differentiating among the multiple human Mu-glutathione S-transferases and molecular cloning of brain GSTM5.多种人类Mu-谷胱甘肽S-转移酶的区分依据及脑GSTM5的分子克隆
J Biol Chem. 1993 Apr 25;268(12):8893-8.
3
Glutathione S-transferases: gene structure and regulation of expression.谷胱甘肽S-转移酶：基因结构与表达调控
Characterization of pig liver glutathione S-transferases using HPLC-electrospray-ionization mass spectrometry.
利用高效液相色谱-电喷雾电离质谱法对猪肝谷胱甘肽S-转移酶进行表征
Biochem J. 1996 Aug 1;317 ( Pt 3)(Pt 3):879-84. doi: 10.1042/bj3170879.
4
Variability of glutathione S-transferase isoenzyme patterns in matched normal and cancer human breast tissue.配对的正常和癌性人乳腺组织中谷胱甘肽S-转移酶同工酶谱的变异性
Biochem J. 1994 Dec 15;304 ( Pt 3)(Pt 3):843-8. doi: 10.1042/bj3040843.
Crit Rev Biochem Mol Biol. 1993;28(3):173-207. doi: 10.3109/10409239309086794.
4
Co-variation of glutathione transferase expression and cytostatic drug resistance in HeLa cells: establishment of class Mu glutathione transferase M3-3 as the dominating isoenzyme.人宫颈癌HeLa细胞中谷胱甘肽转移酶表达与细胞毒性药物耐药性的共变关系：以μ类谷胱甘肽转移酶M3-3作为主要同工酶的确立
Biochem J. 1994 Jan 1;297 ( Pt 1)(Pt 1):59-67. doi: 10.1042/bj2970059.
5
Molecular cloning of the cDNAs for the subunits of rat mitochondrial fatty acid beta-oxidation multienzyme complex. Structural and functional relationships to other mitochondrial and peroxisomal beta-oxidation enzymes.大鼠线粒体脂肪酸β-氧化多酶复合体亚基的cDNA分子克隆。与其他线粒体和过氧化物酶体β-氧化酶的结构和功能关系。
J Biol Chem. 1993 Dec 15;268(35):26452-60.
6
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Biochemistry. 1993 Oct 26;32(42):11405-12. doi: 10.1021/bi00093a018.
7
Variability of glutathione S-transferase isoenzyme patterns in matched normal and cancer human breast tissue.配对的正常和癌性人乳腺组织中谷胱甘肽S-转移酶同工酶谱的变异性
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8
Glutathione transferase (human placenta).谷胱甘肽转移酶（人胎盘）
Methods Enzymol. 1981;77:231-5. doi: 10.1016/s0076-6879(81)77030-7.
9
Fatty acid oxidation complex from Escherichia coli.来自大肠杆菌的脂肪酸氧化复合体。
Methods Enzymol. 1981;71 Pt C:403-11. doi: 10.1016/0076-6879(81)71051-6.
10
[The metabolism of unsaturated fatty acid. 3. On the beta-oxidation of mono- and polyene-fatty acids. The mechanism of the enzymatic reaction on delta-3-cis-enoyl-CoA compounds].[不饱和脂肪酸的代谢。3. 关于单烯和多烯脂肪酸的β-氧化。δ-3-顺式烯酰辅酶A化合物的酶促反应机制]
Hoppe Seylers Z Physiol Chem. 1964;339(1):167-81.