Shiki Y, Shirai K, Saito Y, Yoshida S, Mori Y, Wakashin M
Second Department of Internal Medicine, School of Medicine, Chiba University, Japan.
J Gastroenterol Hepatol. 1992 Jan-Feb;7(1):12-6. doi: 10.1111/j.1440-1746.1992.tb00927.x.
Studies were made on why glycyrrhizin injection decreases the plasma aspartate aminotransferase (AST) and alanine aminotransferase activities in patients with chronic hepatitis. For this, rat hepatocytes were isolated, and incubated with antibody raised against rat liver cell membranes, and the effect of glycyrrhizin on their release of transaminase was investigated. Isolated rat hepatocytes released AST on incubation with anti-liver cell antibody in the presence of complement. At this time, their endogenous phospholipase A2 activity was increased. Cultured hepatocytes also released the transaminase in the presence of venom phospholipase A2. Glycyrrhizin suppressed the release of transaminase in the presence of either anti-liver cell membrane antibody or phospholipase A2. These results suggest that antibody treatment raised the phospholipase A2 activity in liver cell membranes, resulting in release of transaminases, and that glycyrrhizin suppressed this increase in phospholipase A2 activity and so inhibited the release of transaminase.
针对甘草酸注射液降低慢性肝炎患者血浆中天冬氨酸氨基转移酶（AST）和丙氨酸氨基转移酶活性的原因展开了研究。为此，分离出大鼠肝细胞，用抗大鼠肝细胞膜产生的抗体进行孵育，并研究了甘草酸对其转氨酶释放的影响。分离出的大鼠肝细胞在补体存在的情况下与抗肝细胞抗体孵育时会释放AST。此时，其内源性磷脂酶A2活性增加。培养的肝细胞在有蛇毒磷脂酶A2存在时也会释放转氨酶。甘草酸在抗肝细胞膜抗体或磷脂酶A2存在的情况下均能抑制转氨酶的释放。这些结果表明，抗体处理提高了肝细胞膜中的磷脂酶A2活性，导致转氨酶释放，而甘草酸抑制了磷脂酶A2活性的这种增加，从而抑制了转氨酶的释放。
