Kubota H, Morita T, Satta Y, Nozaki M, Matsushiro A
Department of Microbial Genetics, Osaka University, Japan.
Mamm Genome. 1992;2(4):246-51. doi: 10.1007/BF00355434.
We have isolated clones of a processed pseudogene of mouse t complex polypeptide 1 (Tcp-1) and determined the nucleotide sequence of the pseudogene. The pseudogene was 1363 bp long and had no intron. The Tcp-1 pseudogene had 88.4% or 88.3% nucleotide identity to the mouse Tcp-1 cDNA of wild-type (Tcp-1b) or t haplotype (Tcp-1a), and 87.5% identity to the rat Tcp-1 cDNA. On 12 nucleotide positions where the open reading frames (ORFs) of mouse Tcp-1b and Tcp-1a cDNAs have bp substitutions, the Tcp-1 pseudogene had 6 bp identical to Tcp-1b, 5 bp identical to Tcp-1a and 1 bp not identical to neither. On ten amino acid positions where TCP-1B and TCP-1A polypeptides have substitutions, deduced amino acids of the Tcp-1 pseudogene had four amino acids identical to TCP-1B, five amino acids identical to TCP-1A and one amino acid identical to neither. These results suggest that the ancestral mouse Tcp-1 gene would have had no significant difference between the resemblance to Tcp-1b and that to Tcp-1a before they were diverged and that amino acids of TCP-1B and TCP-1A would have been substituted in similar high rates.
我们分离出了小鼠t复合多肽1（Tcp-1）加工假基因的克隆，并确定了该假基因的核苷酸序列。该假基因长1363 bp，无内含子。Tcp-1假基因与野生型（Tcp-1b）或t单倍型（Tcp-1a）的小鼠Tcp-1 cDNA的核苷酸同一性为88.4%或88.3%，与大鼠Tcp-1 cDNA的同一性为87.5%。在小鼠Tcp-1b和Tcp-1a cDNA的开放阅读框（ORF）存在碱基替换的12个核苷酸位置上，Tcp-1假基因有6个碱基与Tcp-1b相同，5个碱基与Tcp-1a相同，1个碱基与两者都不同。在TCP-1B和TCP-1A多肽发生替换的10个氨基酸位置上，Tcp-1假基因推导的氨基酸有4个与TCP-1B相同，5个与TCP-1A相同，1个与两者都不同。这些结果表明，在小鼠Tcp-1基因分化之前，其与Tcp-1b和Tcp-1a的相似性之间没有显著差异，并且TCP-1B和TCP-1A的氨基酸以相似的高频率发生了替换。
