一种黑腹果蝇基因编码一种与小鼠t复合多肽1同源的蛋白质。

A Drosophila melanogaster gene encodes a protein homologous to the mouse t complex polypeptide 1.

作者信息

Ursic D, Ganetzky B

机构信息

Laboratory of Genetics, University of Wisconsin, Madison 53706.

出版信息

Gene. 1988 Sep 7;68(2):267-74. doi: 10.1016/0378-1119(88)90029-7.

DOI:10.1016/0378-1119(88)90029-7

PMID:3146529

Abstract

We have isolated and sequenced a cDNA from Drosophila melanogaster that is homologous to the mouse Tcp-1 gene encoding the t complex polypeptide 1, TCP-1. The Drosophila gene maps by in situ hybridization to bands 94B1-2 of the polytene chromosomes. It shares 66% nucleotide sequence identity with the mouse gene. The predicted Drosophila protein consists of 557 amino acids and shares 72% identity with the mouse polypeptide. The TCP-1 polypeptide appears to be highly conserved in evolution from mammals to simple eukaryotes because the Drosophila gene probe also detects related sequences in DNA from the yeast, Saccharomyces cerevisiae. The presence of TCP-1-related polypeptides in organisms such as Drosophila and yeast should facilitate biochemical and genetic analysis of its function.

摘要

我们从黑腹果蝇中分离并测序了一个与小鼠Tcp-1基因同源的cDNA，该基因编码t复合体多肽1（TCP-1）。通过原位杂交，果蝇基因定位于多线染色体的94B1-2带。它与小鼠基因的核苷酸序列同一性为66%。预测的果蝇蛋白由557个氨基酸组成，与小鼠多肽的同一性为72%。从哺乳动物到简单真核生物，TCP-1多肽在进化过程中似乎高度保守，因为果蝇基因探针也能检测到来自酿酒酵母DNA中的相关序列。在果蝇和酵母等生物体中存在TCP-1相关多肽，这将有助于对其功能进行生化和遗传分析。