基于钌卟啉荧光团配合物的荧光法一氧化氮检测

Fluorescence-based nitric oxide detection by ruthenium porphyrin fluorophore complexes.

作者信息

Lim Mi Hee, Lippard Stephen J

机构信息

Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.

出版信息

Inorg Chem. 2004 Oct 4;43(20):6366-70. doi: 10.1021/ic035418n.

DOI:10.1021/ic035418n

PMID:15446885

Abstract

The ruthenium(II) porphyrin fluorophore complexes [Ru(TPP)(CO)(Ds-R)] (TPP = tetraphenylporphinato dianion; Ds = dansyl; R = imidazole (im), 1, or thiomorpholine (tm), 2) were synthesized and investigated for their ability to detect nitric oxide (NO) based on fluorescence. The X-ray crystal structures of 1 and 2 were determined. The Ds-im or Ds-tm ligand coordinates to an axial site of the ruthenium(II) center through a nitrogen or sulfur atom, respectively. Both exhibit quenched fluorescence when excited at 368 or 345 nm. Displacement of the metal-coordinated fluorophore by NO restores fluorescence within minutes. These observations demonstrate fluorescence-based NO detection using ruthenium porphyrin fluorophore conjugates.

摘要

合成了钌（II）卟啉荧光团配合物[Ru（TPP）（CO）（Ds-R）]（TPP = 四苯基卟吩二价阴离子；Ds = 丹磺酰基；R = 咪唑（im），1，或硫代吗啉（tm），2），并研究了它们基于荧光检测一氧化氮（NO）的能力。测定了1和2的X射线晶体结构。Ds-im或Ds-tm配体分别通过氮原子或硫原子与钌（II）中心的轴向位点配位。当在368或345 nm激发时，两者均表现出荧光猝灭。NO取代金属配位的荧光团会在几分钟内恢复荧光。这些观察结果证明了使用钌卟啉荧光团共轭物进行基于荧光的NO检测。