检测和理解生物学中一氧化氮的作用。
Detecting and understanding the roles of nitric oxide in biology.
机构信息
Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.
出版信息
Inorg Chem. 2010 Jul 19;49(14):6338-48. doi: 10.1021/ic9022757.
Abstract
We are pursuing a dual strategy for investigating the chemistry of nitric oxide as a biological signaling agent. In one approach, metal-based fluorescent sensors for the detection of NO in living cells are evaluated, and a sensor based on a copper fluorescein complex has proved to be a valuable lead compound. Sensors of this class permit identification of NO from both inducible and constitutive forms of nitric oxide synthase and facilitate investigation of different NO functions in response to external stimuli. In the other approach, we employ synthetic model complexes of iron-sulfur clusters to probe their reactivity toward nitric oxide as biomimics of the active sites of iron-sulfur proteins. Our studies reveal that NO disassembles the Fe-S clusters to form dinitrosyl iron complexes.
摘要
我们正在采取双管齐下的策略来研究一氧化氮作为生物信号分子的化学性质。一种方法是评估用于检测活细胞中 NO 的基于金属的荧光传感器，基于铜荧光素复合物的传感器已被证明是一种有价值的先导化合物。此类传感器可以识别诱导型和组成型一氧化氮合酶的 NO，有助于研究不同的 NO 功能对外界刺激的反应。另一种方法是，我们使用铁硫簇的合成模型配合物来探测它们对作为铁硫蛋白活性部位类似物的一氧化氮的反应性。我们的研究表明，NO 会使 Fe-S 簇解体形成双亚硝酰基铁配合物。
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本文引用的文献
1
Analytical chemistry of nitric oxide.
Annu Rev Anal Chem (Palo Alto Calif). 2009;2:409-33. doi: 10.1146/annurev-anchem-060908-155146.
2
NO formation by a catalytically self-sufficient bacterial nitric oxide synthase from Sorangium cellulosum.
Proc Natl Acad Sci U S A. 2009 Sep 22;106(38):16221-6. doi: 10.1073/pnas.0908443106. Epub 2009 Sep 10.
3
Anionic mixed thiolate-sulfide-bridged Roussin's red esters [(NO)2Fe(mu-SR)(mu-S)Fe(NO)2]- (R = Et, Me, Ph): a key intermediate for transformation of dinitrosyl iron complexes (DNICs) to [2Fe-2S] clusters.
Inorg Chem. 2009 Sep 21;48(18):9027-35. doi: 10.1021/ic9012679.
4
A simple and effective strategy to increase the sensitivity of fluorescence probes in living cells.
J Am Chem Soc. 2009 Jul 29;131(29):10189-200. doi: 10.1021/ja902511p.
5
Dinitrosyl iron complexes relevant to Rieske cluster nitrosylation.
J Am Chem Soc. 2009 Jun 17;131(23):7964-5. doi: 10.1021/ja9030159.
6
Dinitrosyl iron complexes with thiolate ligands: physico-chemistry, biochemistry and physiology.
Nitric Oxide. 2009 Aug;21(1):1-13. doi: 10.1016/j.niox.2009.03.005. Epub 2009 Apr 12.
7
Dinitrosyliron complexes and the mechanism(s) of cellular protein nitrosothiol formation from nitric oxide.
Proc Natl Acad Sci U S A. 2009 Mar 24;106(12):4671-6. doi: 10.1073/pnas.0710416106. Epub 2009 Mar 4.
8
Three-coordinate copper(I) amido and aminyl radical complexes.
J Am Chem Soc. 2009 Mar 25;131(11):3878-80. doi: 10.1021/ja809834k.
9
A genetic analysis of nitrosative stress.
Biochemistry. 2009 Feb 3;48(4):792-9. doi: 10.1021/bi801813n.
10
Transformation of dinitrosyl iron complexes [(NO)2Fe(SR)2]- (R = Et, Ph) into [4Fe-4S] Clusters [Fe4S4(SPh)4]2-: relevance to the repair of the nitric oxide-modified ferredoxin [4Fe-4S] clusters.
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