基于反应的小分子荧光探针用于化学选择性生物成像。
Reaction-based small-molecule fluorescent probes for chemoselective bioimaging.
机构信息
Department of Chemistry, University of California, Berkeley, California 94720, USA.
出版信息
Nat Chem. 2012 Dec;4(12):973-84. doi: 10.1038/nchem.1500.
Abstract
The dynamic chemical diversity of elements, ions and molecules that form the basis of life offers both a challenge and an opportunity for study. Small-molecule fluorescent probes can make use of selective, bioorthogonal chemistries to report on specific analytes in cells and in more complex biological specimens. These probes offer powerful reagents to interrogate the physiology and pathology of reactive chemical species in their native environments with minimal perturbation to living systems. This Review presents a survey of tools and tactics for using such probes to detect biologically important chemical analytes. We highlight design criteria for effective chemical tools for use in biological applications as well as gaps for future exploration.
摘要
元素、离子和分子的动态化学多样性构成了生命的基础，既带来了挑战，也提供了研究的机会。小分子荧光探针可以利用选择性、生物正交化学来报告细胞和更复杂生物样本中的特定分析物。这些探针提供了强大的试剂，可以在最小干扰活细胞系统的情况下，原位探测活性化学物质的生理学和病理学。这篇综述介绍了使用这些探针检测生物重要化学分析物的工具和策略。我们强调了用于生物应用的有效化学工具的设计标准，以及未来探索的空白。
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