Lemes-Marques Eneida G, Yano Tomomasa
Instituto Adolfo Lutz, Laboratório Regional de Campinas-rua São Carlos 720, 13035-420, Campinas, SP, Brazil.
FEMS Microbiol Lett. 2004 Oct 1;239(1):63-70. doi: 10.1016/j.femsle.2004.08.021.
The hemolytic, lecithinase or phosphatidylinositol-specific phospholipase C activities of Listeria monocytogenes can be used to differentiate this pathogenic bacteria from L. innocua, apathogenic, frequently isolated from environmental sources and food. However, the interpretation of these characteristics is problematic because of the variation in the expression of virulence factors by L. monocytogenes, which can be influenced by environmental conditions. We used a cheap, simple plate assay to monitor this expression in strains obtained from various sources and grown under different culture conditions. The results were increasingly significant and were obtained adding activated charcoal and different salts to the culture media, and in some cases changing the culture temperature, all with a rigorous control on the process of media sterilization.
单核细胞增生李斯特菌的溶血、卵磷脂酶或磷脂酰肌醇特异性磷脂酶C活性可用于将这种病原菌与无害李斯特菌区分开来，无害李斯特菌无致病性，常从环境来源和食物中分离得到。然而，由于单核细胞增生李斯特菌毒力因子表达的差异（其可受环境条件影响），这些特性的解读存在问题。我们使用一种廉价、简单的平板试验来监测从各种来源获得并在不同培养条件下生长的菌株中的这种表达。在向培养基中添加活性炭和不同盐类，以及在某些情况下改变培养温度后，结果愈发显著，且所有操作都对培养基灭菌过程进行了严格控制。
