Jensen Jesper B, Pedersen Lars H, Hoiby Poul E, Nielsen Lars B, Hansen T P, Folkenberg J R, Riishede J, Noordegraaf Danny, Nielsen Kristian, Carlsen A, Bjarklev A
Research Center COM, Technical University of Denmark, DK-2800 Kgs Lyngby, Denmark.
Opt Lett. 2004 Sep 1;29(17):1974-6. doi: 10.1364/ol.29.001974.
We demonstrate highly efficient evanescent-wave detection of fluorophore-labeled biomolecules in aqueous solutions positioned in the air holes of the microstructured part of a photonic crystal fiber. The air-suspended silica structures located between three neighboring air holes in the cladding crystal guide light with a large fraction of the optical field penetrating into the sample even at wavelengths in the visible range. An effective interaction length of several centimeters is obtained when a sample volume of less than 1 microL is used.
我们展示了在光子晶体光纤微结构部分气孔中放置的水溶液里，对荧光团标记生物分子进行高效的倏逝波检测。包层中位于三个相邻气孔之间的空气悬浮二氧化硅结构能引导光，即使在可见光波长下，也有很大一部分光场穿透到样品中。当使用小于1微升的样品体积时，可获得几厘米的有效相互作用长度。
