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用于基于荧光的灵敏且可靠生物传感的光流体光子晶体光纤平台。

Optofluidic photonic crystal fiber platform for sensitive and reliable fluorescence based biosensing.

作者信息

Moeglen-Paget Baptiste, Perumal Jayakumar, Humbert Georges, Olivo Malini, Dinish U S

机构信息

Xlim Research Institute, UMR 7252, CNRS, Université de Limoges, 123 Avenue Albert Thomas, 87000 Limoges, France.

A*STAR Skin Research Labs (A*SRL), Agency for Science, Technology and Research (A*STAR), 31 Biopolis Way, #07-01 Nanos, Singapore 138669, Republic of Singapore.

出版信息

Biomed Opt Express. 2024 Jun 13;15(7):4281-4291. doi: 10.1364/BOE.527248. eCollection 2024 Jul 1.

DOI:10.1364/BOE.527248
PMID:39022532
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11249680/
Abstract

Biosensing plays a pivotal role in various scientific domains, offering significant contributions to medical diagnostics, environmental monitoring, and biotechnology. Fluorescence biosensing relies on the fluorescence emission from labelled biomolecules to enable sensitive and selective identification and quantification of specific biological targets in various samples. Photonic crystal fibers (PCFs) have led to the development of optofluidic fibers enabling efficient light-liquid interaction within small liquid volume. Herein, we present the development of a user-friendly optofluidic-fiber platform with simple hardware requirements for sensitive and reliable fluorescence biosensing with high measurement repeatability. We demonstrate a sensitivity improvement of the fluorescence emission up to 17 times compared to standard cuvette measurement, with a limit of detection of Cy5 fluorophore as low as 100 pM. The improvement in measurement repeatability is exploited for detecting haptoglobin protein, a relevant biomarker to diagnose several diseases, by using commercially available Cy5 labelled antibodies. The study aims to showcase an optofluidic platform leveraging the benefits provided by optofluidic fibers, which encompass easy light injection, robustness, and high sensitivity.

摘要

生物传感在各个科学领域都发挥着关键作用，为医学诊断、环境监测和生物技术做出了重大贡献。荧光生物传感依赖于标记生物分子的荧光发射，以实现对各种样品中特定生物靶标的灵敏和选择性识别及定量。光子晶体光纤（PCF）推动了光流体光纤的发展，使其能够在小体积液体中实现高效的光-液相互作用。在此，我们展示了一种用户友好型光流体光纤平台的开发，该平台硬件要求简单，可实现灵敏、可靠的荧光生物传感，且测量重复性高。我们证明，与标准比色皿测量相比，荧光发射的灵敏度提高了17倍，Cy5荧光团的检测限低至100 pM。利用测量重复性的提高，通过使用市售的Cy5标记抗体来检测触珠蛋白，这是一种用于诊断多种疾病的相关生物标志物。该研究旨在展示一个利用光流体光纤优势的光流体平台，这些优势包括易于光注入、坚固性和高灵敏度。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0040/11249680/74e6d955e4a7/boe-15-7-4281-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0040/11249680/023c513213b3/boe-15-7-4281-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0040/11249680/a92c8e4a242f/boe-15-7-4281-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0040/11249680/b9785c0bc2f3/boe-15-7-4281-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0040/11249680/32d4567b20c1/boe-15-7-4281-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0040/11249680/b4227c3530a8/boe-15-7-4281-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0040/11249680/74e6d955e4a7/boe-15-7-4281-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0040/11249680/023c513213b3/boe-15-7-4281-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0040/11249680/a92c8e4a242f/boe-15-7-4281-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0040/11249680/b9785c0bc2f3/boe-15-7-4281-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0040/11249680/32d4567b20c1/boe-15-7-4281-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0040/11249680/b4227c3530a8/boe-15-7-4281-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0040/11249680/74e6d955e4a7/boe-15-7-4281-g006.jpg

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本文引用的文献

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Development of a sensitive and reliable ELISA kit of urinary haptoglobin to predict progress of diabetic kidney disease.研发一种灵敏且可靠的尿触珠蛋白 ELISA 试剂盒，用于预测糖尿病肾病的进展。
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Ultrasensitive haptoglobin biomarker detection based on amplified chemiluminescence of magnetite nanoparticles.
基于磁铁纳米粒子放大化学发光的超敏触珠蛋白生物标志物检测
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