肿瘤坏死因子α抗体及乌司他丁对大鼠肝脏缺血再灌注的保护作用
Protective effects of tumor necrosis factor alpha antibody and ulinastatin on liver ischemic reperfusion in rats.
作者信息
Yang Yan-Ling, Li Ji-Peng, Xu Xiao-Ping, Dou Ke-Feng, Yue Shu-Qiang, Li Kai-Zong
机构信息
Department of Hepatobiliary Surgery, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, Shaanxi Province, China.
出版信息
World J Gastroenterol. 2004 Nov 1;10(21):3161-4. doi: 10.3748/wjg.v10.i21.3161.
AIM
To study the protective effects of tumor necrosis factor alpha (TNF alpha) antibody and ulinastatin on liver ischemic reperfusion in rats.
METHODS
One hundred and twenty male SD rats were randomly divided into four groups: normal control group, ischemic group, TNF alpha antibody group and TNF alpha antibody + ulinastatin group. The animals were killed at 0, 3, 6, 9, 12 h after ischemia for 60 min and followed by reperfusion. Serum alanine aminotransferase (ALT), malondialdehyde (MDA) and liver histopathology were observed.
RESULTS
After ischemic reperfusion, the serum ALT and MDA were remarkably increased, and the hepatic congestion was obvious. Treatment of TNF alpha antibody and ulinastatin could significantly decrease serum ALT and MDA levels, and relieve hepatic congestion.
CONCLUSION
Ulinastatin and TNF alpha antibody can suppress the inflammatory reaction induced by hepatic ischemic reperfusion, and have protective effects on rat hepatic ischemic reperfusion injury.
目的
研究肿瘤坏死因子α（TNFα）抗体和乌司他丁对大鼠肝脏缺血再灌注的保护作用。
方法
将120只雄性SD大鼠随机分为四组：正常对照组、缺血组、TNFα抗体组和TNFα抗体+乌司他丁组。缺血60分钟后再灌注，于缺血后0、3、6、9、12小时处死动物，观察血清丙氨酸氨基转移酶（ALT）、丙二醛（MDA）及肝脏组织病理学变化。
结果
缺血再灌注后，血清ALT和MDA显著升高，肝脏淤血明显。TNFα抗体和乌司他丁治疗可显著降低血清ALT和MDA水平，减轻肝脏淤血。
结论
乌司他丁和TNFα抗体可抑制肝脏缺血再灌注诱导的炎症反应，对大鼠肝脏缺血再灌注损伤具有保护作用。
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