Kim Shin-Duk, Nho Hong Joon
Department of Biological Engineering, Seokyeong University, Seoul 136-704, Korea.
J Microbiol. 2004 Sep;42(3):223-7.
An alpha-glucosidase inhibitor, SKG-3, was isolated from the fruiting bodies of Ganoderma lucidum and its physico-chemical properties were characterized. It was a highly specific and effective reversible inhibitor of alpha-glucosidase. It showed very potent inhibitory activity against alpha-glucosidase with an IC50 value of 4.6 micro g/ml, but no activity for any other glycosidases tested. Enzyme activity could be recovered upon dialysis, thus providing evidence for the reversibility of the inhibition. A Lineweaver-Burk plot indicated that the SKG-3 inhibition of alpha-glucosidase was competitive.
从灵芝子实体中分离出一种α-葡萄糖苷酶抑制剂SKG-3，并对其理化性质进行了表征。它是一种高度特异性且有效的α-葡萄糖苷酶可逆抑制剂。它对α-葡萄糖苷酶表现出非常强的抑制活性，IC50值为4.6μg/ml，但对所测试的任何其他糖苷酶均无活性。透析后酶活性可以恢复，从而为抑制作用的可逆性提供了证据。Lineweaver-Burk图表明SKG-3对α-葡萄糖苷酶的抑制作用是竞争性的。
