Zhang Hu, Ouyang Qin, Wen Zhong-hui, Liu Wei-ping, Chen Dai-yun, Li Feng-yuan
Department of Gastroenterology, West China Hospital, Sichuan University, Chengdu 610041, China.
Sichuan Da Xue Xue Bao Yi Xue Ban. 2004 Sep;35(5):630-3.
To investigate the relationship of the expression of GRalpha and GRbeta in the colonic mucosal cell of patients with ulcerative colitis to the efficacy of glucocorticoid (GC) therapy and the intensity of inflammation.
GRalpha expression and GRbeta expression in the colonic mucosal specimens were assessed by means of immunohistochemistry. Then comparative analyses were made on the GRalpha and GRbeta expression between the GC resistant group and GC sensitive group at various levels of inflammation. A normal response group was observed for comparison.
The staining intensities of both GRalpha and GRbeta in colonic mucosal specimens showed significant differences between the GC resistant group, GC sensitive group and normal response group. No association between the expression of GRalpha and GRbeta and the degree of inflammation was found.
These findings suggest that both GRalpha and GRbeta play an important role in the mechanism of the action of GC, and GRbeta functions as a dominant negative inhibitor of GRalpha in there. The expression of GRalpha and GRbeta in the colonic mucosal cell of patients with ulcerative colitis may serve as predictors of glucocorticoid response, but cannot be used as the markers of the severity of inflammation.
探讨溃疡性结肠炎患者结肠黏膜细胞中糖皮质激素受体α（GRα）和糖皮质激素受体β（GRβ）的表达与糖皮质激素（GC）治疗疗效及炎症程度的关系。
采用免疫组织化学方法检测结肠黏膜标本中GRα和GRβ的表达。然后对不同炎症程度的GC抵抗组和GC敏感组之间的GRα和GRβ表达进行比较分析。观察正常反应组作为对照。
结肠黏膜标本中GRα和GRβ的染色强度在GC抵抗组、GC敏感组和正常反应组之间存在显著差异。未发现GRα和GRβ的表达与炎症程度之间存在关联。
这些发现表明，GRα和GRβ在GC的作用机制中均起重要作用，且GRβ在其中作为GRα的显性负性抑制剂发挥作用。溃疡性结肠炎患者结肠黏膜细胞中GRα和GRβ的表达可能作为糖皮质激素反应的预测指标，但不能作为炎症严重程度的标志物。
