Fujishima Sho-ichiro, Takeda Hiroaki, Kawata Sumio, Yamakawa Mitsunori
Department of Pathological Diagnostics, Yamagata University School of Medicine, 2-2-2 Iida-Nishi, Yamagata 990-9585, Japan.
Clin Immunol. 2009 Nov;133(2):208-17. doi: 10.1016/j.clim.2009.07.006. Epub 2009 Jul 31.
The objective of this study was to clarify the relationship between the frequency of infiltrating cells expressing the glucocorticoid receptors (GR) alpha and beta in biopsied colonic mucosa and the glucocorticoid (GC) responsiveness of ulcerative colitis (UC) patients. Active UC patients (n=38) were divided into GC-sensitive and GC-resistant groups. GRbeta(+) cells were significantly higher in the GC-resistant group than in the GC-sensitive and control groups. GRalpha mRNA was expressed in all UC patients, while GRbeta mRNA was expressed in only 1 patient in the GC-sensitive group (n=6) and 7 patients in the GC-resistant group (n=8). Double-positive cells for GRbeta and CD4 or CD19 were frequently observed. The Foxp3(+) cell count was significantly higher in the GC-sensitive group than in the GC-resistant group, but double Foxp3(+)GRbeta(+) cells were not observed. These results indicated that the sensitivity of GC therapy could probably be predicted by immunostaining biopsy specimens for GRbeta and Foxp3.
本研究的目的是阐明活检结肠黏膜中表达糖皮质激素受体（GR）α和β的浸润细胞频率与溃疡性结肠炎（UC）患者糖皮质激素（GC）反应性之间的关系。活动期UC患者（n = 38）被分为GC敏感组和GC抵抗组。GC抵抗组中GRβ(+)细胞显著高于GC敏感组和对照组。所有UC患者均表达GRα mRNA，而GRβ mRNA仅在GC敏感组的1例患者（n = 6）和GC抵抗组的7例患者（n = 8）中表达。经常观察到GRβ与CD4或CD19的双阳性细胞。GC敏感组中Foxp3(+)细胞计数显著高于GC抵抗组，但未观察到双阳性Foxp3(+)GRβ(+)细胞。这些结果表明，通过对活检标本进行GRβ和Foxp3免疫染色可能预测GC治疗的敏感性。
