Pan Guang-dong, Cheng Nan-sheng, Xiong Xian-ze, Liu Jiang-wen, Li Sheng-fu, Zahng Guo-fu, Yan Lü-nan
Department of General Surgery, West China Hospital, Sichuan University, Chengdu 610041, China.
Sichuan Da Xue Xue Bao Yi Xue Ban. 2004 Sep;35(5):658-61.
To investigate the inflammatory regulation of human gallbladder epithelial cells (HGBEC) by peroxisome proliferator activated receptor gamma (PPAR-gamma) ligand ciglitazone.
HGBEC were cultured in medium containing human epidermal growth factor (hEGF). HIL-1beta were added into the ciglitazone groups and inflammatory control groups to make inflammatory model . IL-6 and TNF-alpha concentration in ciglitazone groups and all control groups were measured.
HGBEC were cultured in medium successfully. The inflammatory model was made. The longest duration is 25 d. In inflammatory control groups, cells were edema with unclear cellular membrane and plasmid. In ciglitazone groups, the inflammatory edema of cells were less evident than that in inflammatory control groups, especially in 50 micromol/ml group. The IL-6 concentration in ciglitazone groups is lower than that in control group (P<0.01). The relation between the inhibitory effect and the concentration of ciglitazone is positive correlation.
Ciglitazone that can inhibit the inflammation of HGBEC maybe an effective treatment for acute and chronic cholecystitis.
研究过氧化物酶体增殖物激活受体γ（PPAR-γ）配体噻唑烷二酮对人胆囊上皮细胞（HGBEC）的炎症调节作用。
将HGBEC培养于含人表皮生长因子（hEGF）的培养基中。噻唑烷二酮组和炎症对照组加入IL-1β建立炎症模型。检测噻唑烷二酮组和各对照组中IL-6和TNF-α的浓度。
成功将HGBEC培养于培养基中，建立了炎症模型，最长持续时间为25天。炎症对照组细胞出现水肿，细胞膜和质粒不清。噻唑烷二酮组细胞的炎症水肿比炎症对照组轻，尤其是在50μmol/ml组。噻唑烷二酮组的IL-6浓度低于对照组（P<0.01）。抑制作用与噻唑烷二酮浓度呈正相关。
能抑制HGBEC炎症的噻唑烷二酮可能是治疗急慢性胆囊炎的有效药物。
