Bekman N I, Pomelova V G, Laricheva S Iu, Osin N S
Klin Lab Diagn. 2004 Aug(8):15-20.
The lanthanide fluorescence immunoassay was elaborated for quantitative determination of the total thyroxin T4 in bloodstain dried in filter paper; the fields of its clinical application were defined. The method is based on the hardphase concurrent immunoassay with specific monoclonal antibodies to T4 marked by chelates of europium ions and with conjugate of the T3 heterologous hapten sorbed in plate holes with bovine serum albumin. Measurements of the fluorescence intensity were made by a fluorometer in the time resolution mode. The method ensures the T4 determination in a dry bloodstain with a diameter of 3.4 mm within the concentration range of 0 to 400 nmol/l and with the variation coefficient of below or equal to 15%; the results correlated with the findings of the T4 analysis by the DELFIA Neo T4 set, "Wallac Oy", Finland. The method efficiency was demonstrated for screening and verifying the congenital thyroid deficiency in newborns; it was also confirmed that the method can be used for monitoring the functional thyroid condition in adult patients.
建立了镧系元素荧光免疫分析法，用于定量测定滤纸上干燥血迹中的总甲状腺素T4，并确定了其临床应用领域。该方法基于硬相竞争免疫分析，使用铕离子螯合物标记的针对T4的特异性单克隆抗体，以及吸附在板孔中的T3异源半抗原与牛血清白蛋白的缀合物。通过荧光计在时间分辨模式下测量荧光强度。该方法可确保在浓度范围为0至400 nmol/l、变异系数低于或等于15%的情况下，对直径为3.4 mm的干燥血迹中的T4进行测定；结果与芬兰“Wallac Oy”公司的DELFIA Neo T4试剂盒对T4的分析结果相关。该方法在新生儿先天性甲状腺功能减退症的筛查和验证中显示出有效性；还证实该方法可用于监测成年患者的甲状腺功能状况。
