Fox Irina J, Paucar Andres A, Nakano Ichiro, Mottahedeh Jack, Dougherty Joseph D, Kornblum Harley I
UCLA Interdepartmental Program in Molecular, Cellular, and Integrative Physiology, David Geffen School of Medicine, Los Angeles, CA 90095, USA.
Brain Res Dev Brain Res. 2004 Oct 15;153(1):121-5. doi: 10.1016/j.devbrainres.2004.07.011.
Postnatal neural stem cells (NSCs) express the "traditional" astrocyte marker, glial fibrillary acidic protein (GFAP). Here, we analyze the ontogeny of GFAP mRNA in mouse forebrain germinal zones (GZ). On embryonic day 15, mRNA distribution is highly restricted. Subsequently, expression expands to include many cells in the GZ regions adjacent to the cortex and septum but not to the striatum. Double immunostaining for GFAP and nestin did not demonstrate extensive overlap in the GZ of adult rats, suggesting that either few of the GFAP-expressing cells are stem cells, or that nestin is not a reliable marker for stem cells in the adult rat brain. The current findings indicate that while some GFAP-expressing cells in the GZ may be NSCs, most are not likely to function in a neurogenic capacity.
产后神经干细胞（NSCs）表达“传统的”星形胶质细胞标志物，即胶质纤维酸性蛋白（GFAP）。在此，我们分析了小鼠前脑生发区（GZ）中GFAP mRNA的个体发生。在胚胎第15天，mRNA分布高度受限。随后，表达扩展至包括皮质和隔膜相邻的GZ区域中的许多细胞，但不包括纹状体。对成年大鼠GZ进行GFAP和巢蛋白的双重免疫染色未显示广泛重叠，这表明要么表达GFAP的细胞中很少有干细胞，要么巢蛋白不是成年大鼠脑中干细胞的可靠标志物。目前的研究结果表明，虽然GZ中一些表达GFAP的细胞可能是神经干细胞，但大多数细胞不太可能具有神经发生功能。
