Lang B, Liu H L, Liu R, Feng G D, Jiao X Y, Ju G
Center for Spinal Cord Injury and Repair, Institute of Neurosciences, The Fourth Military Medical University, 17 Chang Le Xi Road, Xi'an 710032, China.
Neuroscience. 2004;128(4):775-83. doi: 10.1016/j.neuroscience.2004.06.033.
It has been well documented that in adult rats astrocytes in the subventricular zone and subgranular layer of the dentate gyrus are neural stem cells. Elsewhere in the CNS astrocytes are not generally recognized as stem cells. Here we describe nestin expression in a population of astrocytes in the spinal cord of adult rat following cord injury. In either hemitransectioned or longitudinally cut spinal cord, there was widespread nestin expression in astrocytes of both the gray and white matters. Isolation of the lateral part of the spinal cord from the central canal region, where stem cells may reside, could not block the appearance of nestin-immunoreactive astrocytes in the lateral cord, and none of them showed Fast DiI labeling after the central canal ependyma had been labeled by the dye, indicating that the nestin-immunoreactive astrocytes can evolve locally in the lateral cord. They were found to be undergoing a process of de-differentiation. Culture of the nestin-immunoreactive astrocytes of the lateral cord generated neurospheres, the cells of which had the ability of self-renewal, and were able to differentiate into neurons, astrocytes, or oligodendrocytes. Taken together, the results indicate that the astrocytes in injured adult rat spinal cord may acquire the potential of neural stem cells.
已有充分文献记载，在成年大鼠中，脑室下区和齿状回颗粒下层的星形胶质细胞是神经干细胞。在中枢神经系统的其他部位，星形胶质细胞一般不被认为是干细胞。在此，我们描述成年大鼠脊髓损伤后一群星形胶质细胞中巢蛋白的表达。在半横断或纵切的脊髓中，灰质和白质的星形胶质细胞中均广泛存在巢蛋白表达。将脊髓外侧部分与可能存在干细胞的中央管区域分离，不能阻止外侧脊髓中巢蛋白免疫反应性星形胶质细胞的出现，并且在用染料标记中央管室管膜后，这些细胞均未显示Fast DiI标记，这表明巢蛋白免疫反应性星形胶质细胞可在外侧脊髓中局部演变。发现它们正在经历去分化过程。外侧脊髓巢蛋白免疫反应性星形胶质细胞的培养产生了神经球，其细胞具有自我更新能力，并能够分化为神经元、星形胶质细胞或少突胶质细胞。综上所述，结果表明成年大鼠损伤脊髓中的星形胶质细胞可能获得神经干细胞的潜能。
