Kofron Michelle D, Li Xudong, Laurencin Cato T
Curr Opin Biotechnol. 2004 Oct;15(5):399-405. doi: 10.1016/j.copbio.2004.07.004.
A tissue engineering approach to bone regeneration includes the use of a scaffold, cells and bioactive factors alone or in various combinations. Several investigators have demonstrated enhanced bone formation when the tissue-engineered construct possesses traits inherent to autogenic bone grafts, namely osteoconductivity, osteoinductivity and osteogenicity. Use of the biodegradable polymer poly(lactide-co-glycolide) in combination with bone morphogenetic protein or primary cells genetically modified to release osteogenic protein have demonstrated the ability to induce osteogenic differentiation of, and subsequent mineralization by, muscle-derived cells and mesenchymal stem cells in both in vitro and in vivo applications.
一种用于骨再生的组织工程方法包括单独或多种组合使用支架、细胞和生物活性因子。一些研究人员已经证明，当组织工程构建体具有自体骨移植所固有的特性，即骨传导性、骨诱导性和成骨性时，骨形成会增强。将可生物降解聚合物聚（丙交酯-共-乙交酯）与骨形态发生蛋白或经过基因改造以释放成骨蛋白的原代细胞联合使用，已证明在体外和体内应用中都能够诱导肌肉来源细胞和间充质干细胞发生成骨分化并随后矿化。
