Laurinavichene Tatyana, Tolstygina Irena, Tsygankov Anatoly
Institute of Basic Biological Problems, Pushchino, Moscow region 142290, Russia.
J Biotechnol. 2004 Oct 19;114(1-2):143-51. doi: 10.1016/j.jbiotec.2004.05.012.
The effect of light intensity on hydrogen production by sulfur-deprived Chlamydomonas reinhardtii was studied in situ using either long- or short-term experiments, or alternatively, with samples withdrawn from the photobioreactor. Overall hydrogen production by S-deprived culture was shown to depend on the light intensity and to exhibit regions of light limitation and light inhibition. The optimal incident light intensity for hydrogen production was independent of the method of sulfur deprivation or the initial acetate concentration in the medium (12-34 mM). However, it varied with the Chl concentration and the thickness of the photobioreactor. To calculate the average light intensity in the photobioreactor under different experimental conditions, a special mathematics approach was developed. The optimal average light intensity for H(2) production appeared to be 30-40 microE m(-2)s(-1) and was independent of the Chl or acetate concentrations and the method of S deprivation. The inhibitory effect of high light intensity was related to the enhanced O(2) evolution activity during the photosynthetic stage of sulfur deprivation and to the high activity of photosystem II at the beginning of the H(2)-production phase. Data support the major role of photosystem II in supplying reductants through photosystem I to the hydrogenase throughout the H(2)-production phase.
利用长期或短期实验，或者从光生物反应器中取出样品，原位研究了光强度对缺硫莱茵衣藻产氢的影响。结果表明，缺硫培养物的总产氢量取决于光强度，并呈现出光限制和光抑制区域。产氢的最佳入射光强度与缺硫方法或培养基中初始乙酸盐浓度（12 - 34 mM）无关。然而，它会随叶绿素浓度和光生物反应器的厚度而变化。为了计算不同实验条件下光生物反应器中的平均光强度，开发了一种特殊的数学方法。产氢的最佳平均光强度似乎为30 - 40 μE m⁻² s⁻¹，且与叶绿素或乙酸盐浓度以及缺硫方法无关。高光强度的抑制作用与缺硫光合阶段氧气释放活性增强以及产氢阶段开始时光系统II的高活性有关。数据支持光系统II在整个产氢阶段通过光系统I向氢化酶供应还原剂的主要作用。
