Tanabayashi K, Takeuchi K, Okazaki K, Hishiyama M, Yamada A
Department of Measles Virus, National Institute of Health, Tokyo, Japan.
Virology. 1992 Apr;187(2):801-4. doi: 10.1016/0042-6822(92)90482-5.
Two recombinant plasmids were constructed by inserting the cDNAs of either the fusion (F) or the hemagglutinin-neuraminidase (HN) protein genes of mumps virus into the pcDL-SR alpha expression vector. Both the F and the HN proteins expressed in COS7 cells transfected with their respective recombinant plasmids were indistinguishable in terms of electrophoretic mobility from their counterparts synthesized in mumps virus-infected cells. The F protein was cleaved and expressed on the cell surface, but uncleaved forms were also detected. The expressed HN protein was transported to the cell surface and adsorbed guinea pig erythrocytes. Syncytium formation was induced when COS7 cells were transfected with both recombinant plasmid DNAs together, but not with the recombinant plasmid only carrying the F gene. This observation indicates that cell fusion mediated by mumps virus requires both the F and the HN glycoproteins.
通过将腮腺炎病毒的融合（F）蛋白基因或血凝素神经氨酸酶（HN）蛋白基因的cDNA插入pcDL-SRα表达载体，构建了两种重组质粒。在用各自重组质粒转染的COS7细胞中表达的F蛋白和HN蛋白，其电泳迁移率与在腮腺炎病毒感染细胞中合成的对应蛋白没有区别。F蛋白被切割并表达在细胞表面，但也检测到未切割的形式。表达的HN蛋白被转运到细胞表面并吸附豚鼠红细胞。当COS7细胞同时用两种重组质粒DNA转染时可诱导形成多核巨细胞，但仅用携带F基因的重组质粒转染则不会。这一观察结果表明，腮腺炎病毒介导的细胞融合需要F糖蛋白和HN糖蛋白两者。
