Piérard D, Vandenbussche H, Verschraegen I, Lauwers S
Département de Microbiologie, Academisch Ziekenhuis Vrije Universiteit Brussel, Laarbeeklaan 101, B-1090 Bruxelles, Belgium.
Pathol Biol (Paris). 2004 Oct;52(8):486-8. doi: 10.1016/j.patbio.2004.07.016.
The aim of the present study was to investigate the prevalence of vancomycin resistance in clinical methicillin resistant Staphylococcus aureus (MRSA) isolates in our hospital by screening on Mueller-Hinton agar with 5 mg/l teicoplanin (MH-Teico), as recommended by the Comite de l'Antibiogramme of the Societe Francaise de Microbiologie (CA-SFM). Seventeen of 1002 clinical MRSA isolated from 404 patients showed in 2003 growth of at least four colonies on this medium, but only one was confirmed as homogeneous vancomycin-resistant S. aureus (VISA) and five as heterogeneous VISA (hVISA) by population analysis. None of the patients presented with severe infection but awareness is needed and screening on MH-Teico as recommended by CA-SFM is a convenient method. Surveillance should be focused on patients with risk factors for selection of such strains: patients with a prolonged course of glycopeptide therapy and persistence of MRSA infection or colonization.
本研究的目的是按照法国微生物学会抗生素药敏试验委员会(CA - SFM)的建议,通过在含5 mg/l替考拉宁的穆勒 - 欣顿琼脂(MH - Teico)上进行筛选,调查我院临床耐甲氧西林金黄色葡萄球菌(MRSA)分离株中万古霉素耐药的发生率。2003年,从404例患者中分离出的1002株临床MRSA中有17株在此培养基上生长出至少四个菌落,但通过群体分析,只有1株被确认为同源性耐万古霉素金黄色葡萄球菌(VISA),5株为异质性VISA(hVISA)。所有患者均无严重感染,但仍需提高警惕,按照CA - SFM的建议在MH - Teico上进行筛选是一种便捷的方法。监测应集中于有此类菌株选择危险因素的患者:糖肽类治疗疗程延长且MRSA感染或定植持续存在的患者。