Su Yan-Lei, Li Jin-Ru, Jiang Long
Key Laboratory of Colloid and Interface Science, Center for Molecular Science, Institute of Chemistry, Chinese Academy of Science, Beijing 100080, PR China.
Colloids Surf B Biointerfaces. 2004 Oct 10;38(1-2):29-33. doi: 10.1016/j.colsurfb.2004.08.010.
A new approach of chromatic immunoassay based on polydiacetylene vesicles is described. Antibodies were covalently coupled with mixed vesicles of 10,12-tricosadiynoic acid (TCDA) and dimyristoylphosphatidycholine (DMPC). The vesicle-antibody conjugates were irradiated with UV light to yield a blue-colored polydiacetylene. After antigen injection, specific immunoreactions took place at the vesicle surface alter polydiacetylene conformation and lead to a color change from blue to red. The chromatic immunoassay described here is simple, rapid, sensitive; the color change was readily discernible by naked eye when the concentration of antigen is 1 ng/mL. Incorporation of DMPC in the mixed vesicles increases the sensitivity of the chromatic immunoassay.
本文描述了一种基于聚二乙炔囊泡的彩色免疫分析新方法。抗体与10,12-二十三碳二炔酸（TCDA）和二肉豆蔻酰磷脂酰胆碱（DMPC）的混合囊泡共价偶联。用紫外光照射囊泡-抗体偶联物，产生蓝色聚二乙炔。注射抗原后，特异性免疫反应在囊泡表面发生，改变聚二乙炔构象，导致颜色从蓝色变为红色。本文所述的彩色免疫分析方法简单、快速、灵敏；当抗原浓度为1 ng/mL时，肉眼即可轻松辨别颜色变化。在混合囊泡中加入DMPC可提高彩色免疫分析的灵敏度。
