Sayer G L, Smith P D C
Department of Vascular Surgery, King's College Hospital, London SE5 9RS, UK.
Eur J Vasc Endovasc Surg. 2004 Nov;28(5):479-83. doi: 10.1016/j.ejvs.2004.07.023.
To characterise the inflammatory cell infiltrate in varicose vein wall, and its relationship to the valve complex.
A comparative study of the distribution of inflammatory cells in normal and varicose vein.
Specimens of proximal human long saphenous vein were obtained from patients with duplex Doppler confirmed long saphenous vein reflux (n=14). Control vein was obtained from patients undergoing coronary artery bypass without clinical evidence of venous insufficiency (n=6). Longitudinal 7 microm frozen sections of vein, displaying valve, were prepared.
Using immunohistochemistry, T-lymphocytes (CD3), macrophage/monocytes (CD68), neutrophils (CD15s) and mast cells (anti-mast cell tryptase) were identified. The number of cells per unit length vein were counted using light microscopy.
There were significantly more mast cells and macrophage/monocytes in varicose vein as compared to control. There was a non-significant trend towards more T-lymphocytes in varicose vein. Few neutrophils were present in varicose or normal vein. The distribution of inflammatory cells with respect to the valve was not found to be significant.
Varicose veins display a greater inflammatory cell infiltrate than normal vein. The key role of macrophage/monocytes and mast cells in tissue damage and remodelling should stimulate further research into whether they play a significant role in the development of chronic venous insufficiency.
描述静脉曲张壁中的炎性细胞浸润情况及其与瓣膜复合体的关系。
对正常静脉和静脉曲张中炎性细胞分布进行对比研究。
从经双功多普勒证实有大隐静脉反流的患者（n = 14）获取人近端大隐静脉标本。对照静脉取自无静脉功能不全临床证据的冠状动脉搭桥手术患者（n = 6）。制备显示瓣膜的静脉纵向7微米冰冻切片。
采用免疫组织化学方法鉴定T淋巴细胞（CD3）、巨噬细胞/单核细胞（CD68）、中性粒细胞（CD15s）和肥大细胞（抗肥大细胞类胰蛋白酶）。使用光学显微镜计数每单位长度静脉中的细胞数量。
与对照相比，静脉曲张中的肥大细胞和巨噬细胞/单核细胞明显更多。静脉曲张中T淋巴细胞数量有增多的趋势，但无统计学意义。在静脉曲张或正常静脉中很少有中性粒细胞。未发现炎性细胞相对于瓣膜的分布有统计学意义。
静脉曲张比正常静脉表现出更多的炎性细胞浸润。巨噬细胞/单核细胞和肥大细胞在组织损伤和重塑中的关键作用应促使进一步研究它们在慢性静脉功能不全发展中是否起重要作用。
