Cejas Juana Rosa, Almansa Eduardo, Jérez Salvador, Bolaños Ana, Felipe Beatriz, Lorenzo Antonio
Centro Oceanográfico de Canarias (I.E.O.). Crta. de San Andrés s/n, 38120 Santa Cruz de Tenerife, Spain.
Comp Biochem Physiol B Biochem Mol Biol. 2004 Oct;139(2):209-16. doi: 10.1016/j.cbpc.2004.07.010.
To establish the changes which occur during embryogenesis and early larvae development, eggs, yolk-sac larvae (one day old larvae) and absorbed yolk-sac larvae (three day old larvae) of white sea bream were examined for lipid class and fatty acid composition. The development was characterized by a decrease in all lipid classes with the exception of phosphatidylserine (PS) and fatty free acids (FFA) which increased, and sphingomyelin (SM) which remained unchanged. The changes observed in lipid class content and the decrease in fatty acids in total lipid (TL) reflect the utilization and mobilization of lipids during both embryogenesis and early larvae development. Fluctuations in the relative composition of fatty acids in phosphatidylcholine (PC) during development suggest a selective bulk uptake and catabolism of fatty acids in this lipid class. Unlike PC, catabolism of triacylglycerol (TG) fatty acid appears to be non-selective. During development, the decrease in levels of polyunsaturated fatty acids (PUFA) eicosapentaenoic (20:5n-3, EPA) and docosahexaenoic (22:6n-3, DHA) in total lipid denotes their utilization as energy substrate by Diplodus sargus larvae.
为确定胚胎发生和早期幼体发育过程中发生的变化，对白鲷的鱼卵、卵黄囊幼体（1日龄幼体）和卵黄囊吸收幼体（3日龄幼体）的脂质类别和脂肪酸组成进行了检测。发育的特征是，除磷脂酰丝氨酸（PS）和游离脂肪酸（FFA）增加、鞘磷脂（SM）保持不变外，所有脂质类别均减少。脂质类别含量的变化以及总脂质（TL）中脂肪酸的减少反映了胚胎发生和早期幼体发育过程中脂质的利用和动员情况。发育过程中磷脂酰胆碱（PC）中脂肪酸相对组成的波动表明该脂质类别中脂肪酸存在选择性的大量摄取和分解代谢。与PC不同，三酰甘油（TG）脂肪酸的分解代谢似乎是非选择性的。在发育过程中，总脂质中多不饱和脂肪酸（PUFA）二十碳五烯酸（20:5n-3，EPA）和二十二碳六烯酸（22:6n-3，DHA）水平的下降表明它们被黑棘鲷幼体用作能量底物。
