铜绿假单胞菌中多聚腺苷酸化mRNA的证据。
Evidence for polyadenylated mRNA in Pseudomonas aeruginosa.
作者信息
Saravanamuthu Senthil Selvan, von Götz Franz, Salunkhe Prabhakar, Chozhavendan Rathinam, Geffers Robert, Buer Jan, Tümmler Burkhard, Steinmetz Ivo
机构信息
Institute for Medical Microbiology and Hospital Epidemiology, Hannover, Germany.
出版信息
J Bacteriol. 2004 Oct;186(20):7015-8. doi: 10.1128/JB.186.20.7015-7018.2004.
Abstract
In this paper, we report the synthesis of Pseudomonas aeruginosa cDNA in the presence of oligo(dT) primers. Hybridization of oligonucleotide DNA microarrays indicates that under the experimental conditions used, at least 43.7% of the expressed genes from P. aeruginosa PAO1, representing many different functional classes, can be detected by using oligo(dT)-primed cDNAs.
摘要
在本文中，我们报道了在寡聚（dT）引物存在的情况下铜绿假单胞菌cDNA的合成。寡核苷酸DNA微阵列的杂交表明，在所使用的实验条件下，使用寡聚（dT）引发的cDNA可以检测到来自铜绿假单胞菌PAO1的至少43.7%的表达基因，这些基因代表了许多不同的功能类别。
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