乳酸乳杆菌属菌株MONT4基因组上具有新型结构组织的两个乳酸脱氢酶基因的分子特征分析
Molecular characterization of two lactate dehydrogenase genes with a novel structural organization on the genome of Lactobacillus sp. strain MONT4.
作者信息
Weekes Jennifer, Yüksel Gülhan U
机构信息
Department of Food Science and Toxicology, University of Idaho, Moscow, ID 83844-2312, USA.
出版信息
Appl Environ Microbiol. 2004 Oct;70(10):6290-5. doi: 10.1128/AEM.70.10.6290-6295.2004.
Abstract
Two lactate dehydrogenase (ldh) genes from Lactobacillus sp. strain MONT4 were cloned by complementation in Escherichia coli DC1368 (ldh pfl) and were sequenced. The sequence analysis revealed a novel genomic organization of the ldh genes. Subcloning of the individual ldh genes and their Northern blot analyses indicated that the genes are monocistronic.
摘要
通过在大肠杆菌DC1368（ldh pfl）中进行互补克隆，从蒙氏乳杆菌MONT4菌株中获得了两个乳酸脱氢酶（ldh）基因，并对其进行了测序。序列分析揭示了ldh基因的一种新的基因组组织形式。对各个ldh基因进行亚克隆并进行Northern印迹分析表明，这些基因是单顺反子的。
相似文献
1
Molecular characterization of two lactate dehydrogenase genes with a novel structural organization on the genome of Lactobacillus sp. strain MONT4.乳酸乳杆菌属菌株MONT4基因组上具有新型结构组织的两个乳酸脱氢酶基因的分子特征分析
Appl Environ Microbiol. 2004 Oct;70(10):6290-5. doi: 10.1128/AEM.70.10.6290-6295.2004.
2
Cloning of the D-lactate dehydrogenase gene from Lactobacillus delbrueckii subsp. bulgaricus by complementation in Escherichia coli.通过在大肠杆菌中互补克隆德氏乳杆菌保加利亚亚种的D-乳酸脱氢酶基因。
FEBS Lett. 1991 Sep 23;290(1-2):61-4. doi: 10.1016/0014-5793(91)81226-x.
3
Lactobacillus plantarum ldhL gene: overexpression and deletion.植物乳杆菌ldhL基因：过表达与缺失
J Bacteriol. 1994 Feb;176(3):596-601. doi: 10.1128/jb.176.3.596-601.1994.
4
Cloning and overexpression of Lactobacillus helveticus D-lactate dehydrogenase gene in Escherichia coli.瑞士乳杆菌D-乳酸脱氢酶基因在大肠杆菌中的克隆与过表达
Eur J Biochem. 1992 Sep 15;208(3):799-805. doi: 10.1111/j.1432-1033.1992.tb17250.x.
5
[Cloning and function analysis of L-lactate dehydrogenase gene from Lactobacillus sp. MD-1].[嗜酸乳杆菌MD-1中L-乳酸脱氢酶基因的克隆及功能分析]
Sheng Wu Gong Cheng Xue Bao. 2004 Sep;20(5):725-9.
6
Cloning and overexpression of the Lactobacillus bulgaricus NAD(+)-dependent D-lactate dehydrogenase gene in Escherichia coli:purification and characterization of the recombinant enzyme.
Biochem Biophys Res Commun. 1992 Jun 15;185(2):705-12. doi: 10.1016/0006-291x(92)91683-h.
7
Cloning, characterization and insertional inactivation of the Lactobacillus helveticus D(-) lactate dehydrogenase gene.瑞士乳杆菌D(-)乳酸脱氢酶基因的克隆、特性分析及插入失活
Appl Microbiol Biotechnol. 1994 Jun;41(4):432-9. doi: 10.1007/BF00939032.
8
Cloning and nucleotide sequencing of L-lactate dehydrogenase gene from Streptococcus thermophilus M-192.
Biosci Biotechnol Biochem. 1994 Sep;58(9):1569-73. doi: 10.1271/bbb.58.1569.
9
Cloning of the L-lactate dehydrogenase gene from the ruminal bacterium Selenomonas ruminantium HD4.从瘤胃细菌反刍月形单胞菌HD4中克隆L-乳酸脱氢酶基因。
Curr Microbiol. 2002 Mar;44(3):155-60. doi: 10.1007/s00284-001-0082-9.
10
Cloning and characterization of l-lactate dehydrogenase gene of Staphylococcus aureus.金黄色葡萄球菌 l-乳酸脱氢酶基因的克隆与特性分析。
Anaerobe. 2013 Dec;24:43-8. doi: 10.1016/j.anaerobe.2013.09.003. Epub 2013 Sep 13.
引用本文的文献
1
CRISPR: a journey of gene-editing based medicine.CRISPR：基因编辑医学之旅。
Genes Genomics. 2020 Dec;42(12):1369-1380. doi: 10.1007/s13258-020-01002-x. Epub 2020 Oct 22.
本文引用的文献
1
Complete genome sequence of Lactobacillus plantarum WCFS1.植物乳杆菌WCFS1的全基因组序列
Proc Natl Acad Sci U S A. 2003 Feb 18;100(4):1990-5. doi: 10.1073/pnas.0337704100. Epub 2003 Feb 3.
2
Cloning of the L-lactate dehydrogenase gene from the ruminal bacterium Selenomonas ruminantium HD4.从瘤胃细菌反刍月形单胞菌HD4中克隆L-乳酸脱氢酶基因。
Curr Microbiol. 2002 Mar;44(3):155-60. doi: 10.1007/s00284-001-0082-9.
3
A case of septicemia with Pediococcus acidilactici after long-term antibiotic treatment.长期抗生素治疗后发生嗜酸片球菌败血症1例。
Eur J Clin Microbiol Infect Dis. 2000 Dec;19(12):946-8. doi: 10.1007/s100960000409.
4
D-Lactate dehydrogenase gene (ldhD) inactivation and resulting metabolic effects in the Lactobacillus johnsonii strains La1 and N312.约氏乳杆菌菌株La1和N312中D-乳酸脱氢酶基因（ldhD）的失活及其产生的代谢效应
Appl Environ Microbiol. 1999 Sep;65(9):4002-7. doi: 10.1128/AEM.65.9.4002-4007.1999.
5
Disruption of the sole ldhL gene in Lactobacillus sakei prevents the production of both L- and D-lactate.
Microbiology (Reading). 1998 Dec;144 ( Pt 12):3327-3333. doi: 10.1099/00221287-144-12-3327.
6
Cloning of the Lactococcus lactis adhE gene, encoding a multifunctional alcohol dehydrogenase, by complementation of a fermentative mutant of Escherichia coli.通过互补大肠杆菌发酵突变体克隆乳酸乳球菌adhE基因，该基因编码一种多功能乙醇脱氢酶。
J Bacteriol. 1998 Jun;180(12):3049-55. doi: 10.1128/JB.180.12.3049-3055.1998.
7
Molecular genetic characterization of the L-lactate dehydrogenase gene (ldhL) of Lactobacillus helveticus and biochemical characterization of the enzyme.瑞士乳杆菌L-乳酸脱氢酶基因（ldhL）的分子遗传学特征及该酶的生化特性
Appl Environ Microbiol. 1997 Jul;63(7):2850-6. doi: 10.1128/aem.63.7.2850-2856.1997.
8
Cloning, sequence, and expression of the L-(+) lactate dehydrogenase of Streptococcus bovis.牛链球菌L-(+)乳酸脱氢酶的克隆、序列测定及表达
Curr Microbiol. 1997 Jun;34(6):367-73. doi: 10.1007/s002849900197.
9
The ldh phylogeny for environmental isolates of Lactococcus lactis is consistent with rRNA genotypes but not with phenotypes.乳酸乳球菌环境分离株的乳酸脱氢酶系统发育与核糖体RNA基因型一致，但与表型不一致。
Appl Environ Microbiol. 1997 Feb;63(2):694-702. doi: 10.1128/aem.63.2.694-702.1997.
10
Identification of a novel operon in Lactococcus lactis encoding three enzymes for lactic acid synthesis: phosphofructokinase, pyruvate kinase, and lactate dehydrogenase.在乳酸乳球菌中鉴定出一个新的操纵子，其编码用于乳酸合成的三种酶：磷酸果糖激酶、丙酮酸激酶和乳酸脱氢酶。
J Bacteriol. 1993 May;175(9):2541-51. doi: 10.1128/jb.175.9.2541-2551.1993.
Zotero 插件