来自黄色粘球菌422的编码凝乳蛋白酶的clt基因的克隆与表达。
Cloning and expression of clt genes encoding milk-clotting proteases from Myxococcus xanthus 422.
作者信息
Poza M, Prieto-Alcedo M, Sieiro C, Villa T G
机构信息
Department of Microbiology and Parasitology, Faculty of Pharmacy, Campus Sur 15782, Santiago de Compostela, Spain.
出版信息
Appl Environ Microbiol. 2004 Oct;70(10):6337-41. doi: 10.1128/AEM.70.10.6337-6341.2004.
Abstract
The screening of a gene library of the milk-clotting strain Myxococcus xanthus 422 constructed in Escherichia coli allowed the description of eight positive clones containing 26 open reading frames. Only three of them (cltA, cltB, and cltC) encoded proteins that exhibited intracellular milk-clotting ability in E. coli, Saccharomyces cerevisiae, and Pichia pastoris expression systems.
摘要
对构建于大肠杆菌中的凝乳菌株黄色粘球菌422的基因文库进行筛选，鉴定出8个阳性克隆，其中包含26个开放阅读框。其中只有3个（cltA、cltB和cltC）编码的蛋白质在大肠杆菌、酿酒酵母和毕赤酵母表达系统中表现出细胞内凝乳能力。
相似文献
1
Cloning and expression of clt genes encoding milk-clotting proteases from Myxococcus xanthus 422.来自黄色粘球菌422的编码凝乳蛋白酶的clt基因的克隆与表达。
Appl Environ Microbiol. 2004 Oct;70(10):6337-41. doi: 10.1128/AEM.70.10.6337-6341.2004.
2
Identification and characterization of the Myxococcus xanthus argE gene.黄色粘球菌argE基因的鉴定与特性分析
J Bacteriol. 1998 Dec;180(23):6412-4. doi: 10.1128/JB.180.23.6412-6414.1998.
3
A nonessential signal peptidase II (Lsp) of Myxococcus xanthus might be involved in biosynthesis of the polyketide antibiotic TA.黄色黏球菌的一种非必需信号肽酶II（Lsp）可能参与聚酮类抗生素TA的生物合成。
J Bacteriol. 1999 Sep;181(18):5644-51. doi: 10.1128/JB.181.18.5644-5651.1999.
4
The lonD gene is homologous to the lon gene encoding an ATP-dependent protease and is essential for the development of Myxococcus xanthus.lonD基因与编码一种ATP依赖性蛋白酶的lon基因同源，并且对于黄色粘球菌的发育至关重要。
J Bacteriol. 1993 Jul;175(14):4545-9. doi: 10.1128/jb.175.14.4545-4549.1993.
5
Photolyase of Myxococcus xanthus, a Gram-negative eubacterium, is more similar to photolyases found in Archaea and "higher" eukaryotes than to photolyases of other eubacteria.革兰氏阴性真细菌黄色黏球菌的光解酶，与古细菌和“高等”真核生物中发现的光解酶相比，与其他真细菌的光解酶更为相似。
J Biol Chem. 1996 Mar 15;271(11):6252-9. doi: 10.1074/jbc.271.11.6252.
6
Protein purification, gene cloning and sequencing of an acidic endoprotease from Myxococcus xanthus DK101.来自黄色粘球菌DK101的酸性内切蛋白酶的蛋白质纯化、基因克隆及测序
Eur J Biochem. 1994 Jun 1;222(2):247-54. doi: 10.1111/j.1432-1033.1994.tb18863.x.
7
Myxococcus xanthus encodes an ATP-dependent protease which is required for developmental gene transcription and intercellular signaling.黄色黏球菌编码一种依赖ATP的蛋白酶，它是发育基因转录和细胞间信号传导所必需的。
J Bacteriol. 1993 Jul;175(14):4538-44. doi: 10.1128/jb.175.14.4538-4544.1993.
8
A novel gene from Myxococcus xanthus that facilitates membrane translocation of an extracellular endoglucanase in Escherichia coli?
Res Microbiol. 2001 Jun;152(5):487-92. doi: 10.1016/s0923-2508(01)01222-0.
9
A Myxococcus xanthus CbpB containing two cAMP-binding domains is involved in temperature and osmotic tolerances.含有两个环磷酸腺苷结合结构域的黄色粘球菌CbpB与温度和渗透压耐受性有关。
FEMS Microbiol Lett. 2005 Mar 1;244(1):75-83. doi: 10.1016/j.femsle.2005.01.027.
10
Molecular cloning of the GTP-cyclohydrolase structural gene RIB1 of Pichia guilliermondii involved in riboflavin biosynthesis.参与核黄素生物合成的季也蒙毕赤酵母GTP-环化水解酶结构基因RIB1的分子克隆
Yeast. 1995 Aug;11(10):945-52. doi: 10.1002/yea.320111005.
引用本文的文献
1
Myxobacteria: Versatile cell factories of novel commercial enzymes for bio-manufacturing.粘细菌：用于生物制造的新型商业酶的多功能细胞工厂。
Biotechnol Adv. 2025 Sep;82:108594. doi: 10.1016/j.biotechadv.2025.108594. Epub 2025 May 8.
2
Cloning and expression of an active aspartic proteinase from Mucor circinelloides in Pichia pastoris.从卷枝毛霉中克隆和表达一种活性天冬氨酸蛋白酶到毕赤酵母中。
BMC Microbiol. 2013 Nov 9;13:250. doi: 10.1186/1471-2180-13-250.
3
Constitutive expression, purification and characterization of bovine prochymosin in Pichia pastoris GS115.毕赤酵母 GS115 中牛凝乳酶原的组成型表达、纯化和特性分析。
World J Microbiol Biotechnol. 2012 May;28(5):2087-93. doi: 10.1007/s11274-012-1012-7. Epub 2012 Feb 10.
4
Proteins associated with the Myxococcus xanthus extracellular matrix.与黄色粘球菌细胞外基质相关的蛋白质。
J Bacteriol. 2007 Nov;189(21):7634-42. doi: 10.1128/JB.01007-07. Epub 2007 Aug 31.
本文引用的文献
1
Production and characterization of the milk-clotting protease of Myxococcus xanthus strain 422.黄色粘球菌422菌株凝乳蛋白酶的产生及特性研究
J Ind Microbiol Biotechnol. 2003 Dec;30(12):691-8. doi: 10.1007/s10295-003-0100-y. Epub 2003 Nov 22.
2
Peptides identified during Emmental cheese ripening: origin and proteolytic systems involved.在埃门塔尔奶酪成熟过程中鉴定出的肽：起源及相关蛋白水解系统
J Agric Food Chem. 2001 Sep;49(9):4402-13. doi: 10.1021/jf000895z.
3
Molecular cloning and targeted deletion of PEP2 which encodes a novel aspartic proteinase from Aspergillus fumigatus.编码烟曲霉新型天冬氨酸蛋白酶的PEP2的分子克隆与靶向缺失
Int J Med Microbiol. 2000 Mar;290(1):85-96. doi: 10.1016/S1438-4221(00)80111-3.
4
Processing, activity, and inhibition of recombinant cyprosin, an aspartic proteinase from cardoon (Cynara cardunculus).来自刺菜蓟（Cynara cardunculus）的天冬氨酸蛋白酶——重组刺菜蓟蛋白酶的加工、活性及抑制作用
J Biol Chem. 1999 Jun 11;274(24):16685-93. doi: 10.1074/jbc.274.24.16685.
5
Cloning and characterization of an endo-beta-1,3(4)glucanase and an aspartic protease from Phaffia rhodozyma CBS 6938.
Appl Microbiol Biotechnol. 1999 Feb;51(2):215-22. doi: 10.1007/s002530051384.
6
Controls of the expression of aspA, the aspartyl protease gene from Penicillium roqueforti.
Mol Gen Genet. 1997 Nov;256(5):557-65. doi: 10.1007/s004380050601.
7
Cloning and sequencing of two genes, prtA and prtB, from Myxococcus xanthus, encoding PrtA and PrtB proteases, both of which are required for the protease activity.
Gene. 1997 Oct 1;198(1-2):135-40. doi: 10.1016/s0378-1119(97)00303-x.
8
Gapped BLAST and PSI-BLAST: a new generation of protein database search programs.空位BLAST和位置特异性迭代BLAST：新一代蛋白质数据库搜索程序。
Nucleic Acids Res. 1997 Sep 1;25(17):3389-402. doi: 10.1093/nar/25.17.3389.
9
Recent advances in the social and developmental biology of the myxobacteria.粘细菌社会与发育生物学的最新进展
Microbiol Rev. 1996 Mar;60(1):70-102. doi: 10.1128/mr.60.1.70-102.1996.
10
Expression and secretion of recombinant aspartic proteinases by Bacillus brevis.
Adv Exp Med Biol. 1995;362:589-96. doi: 10.1007/978-1-4615-1871-6_79.
Zotero 插件