O'Connor K A, McBride M J, West M, Yu H, Trinh L, Yuan K, Lee T, Zusman D R
Department of Molecular and Cell Biology, University of California, Berkeley 94720-3204, USA.
J Biol Chem. 1996 Mar 15;271(11):6252-9. doi: 10.1074/jbc.271.11.6252.
We report the identification of the gene encoding a DNA photolyase (phrA) from the Gram-negative eubacterium Myxococcus xanthus. The deduced amino acid sequence of M. xanthus photolyase indicates that the protein contains 401 amino acids (Mr 45,071). By comparison of the amino acid and DNA sequences with those of other known photolyases, it has been found that it is more similar to the deduced amino acid sequences of the photolyases of "higher" eukaryotes than to the photolyases of other eubacteria. Recombinant plasmids carrying M. xanthus phrA rescue the photoreactivation activity of an irradiated strain of Escherichia coli with a deletion in phrA. This rescue is light-dependent.
我们报道了从革兰氏阴性真细菌黄色粘球菌中鉴定出编码一种DNA光解酶（phrA）的基因。黄色粘球菌光解酶的推导氨基酸序列表明该蛋白质含有401个氨基酸（分子量45,071）。通过将氨基酸和DNA序列与其他已知光解酶的序列进行比较，发现它与“高等”真核生物光解酶的推导氨基酸序列比与其他真细菌的光解酶更相似。携带黄色粘球菌phrA的重组质粒挽救了phrA缺失的经辐射的大肠杆菌菌株的光复活活性。这种挽救是光依赖性的。
