Murata Yoshifumi, Kudo Sayuri, Kofuji Kyouko, Miyamoto Etsuko, Kawashima Susumu
Faculty of Pharmaceutical Science, Hokuriku University, Kanazawa, Japan.
Chem Pharm Bull (Tokyo). 2004 Oct;52(10):1183-5. doi: 10.1248/cpb.52.1183.
The orotic acid (OT) salt of chitosan (CS), CS-OT, and that of a CS derivative, CP, were prepared, and the adsorption of primary or secondary bile acid was investigated. Calcium-induced alginate gel beads (Alg-Ca) containing CS-OT were also prepared and autoclaved, and the possibility of these beads to act as a vehicle for oral administration to prevent hyperlipidemia was investigated. When taurocholate (TCA) and glycocholate (GCA) were present together in the medium, CS-OT adsorbed identical amounts of both bile acids. This trend was seen in all CPs, although the capacity to adsorb bile acid was affected by the number and/or structure of the amino groups in the CP. On the other hand, taurodeoxycholate, a secondary bile acid was preferentially adsorbed over TCA and GCA. Alg-Ca containing CS-OT took up bile acids in a similar manner as CS-OT irrespective of the water content of the gel matrix. As all elements can be taken as a food, Alg-Ca containing CS-OT could serve as a useful dietary agent for the prevention of hyperlipidemia, which is a lifestyle-related disease.
制备了壳聚糖（CS）的乳清酸（OT）盐（CS-OT）以及CS衍生物CP的乳清酸盐，并研究了它们对一级或二级胆汁酸的吸附作用。还制备了含有CS-OT的钙诱导海藻酸盐凝胶珠（Alg-Ca）并进行了高压灭菌，研究了这些珠子作为口服给药载体预防高脂血症的可能性。当牛磺胆酸盐（TCA）和甘氨胆酸盐（GCA）同时存在于培养基中时，CS-OT对两种胆汁酸的吸附量相同。所有CP都呈现出这种趋势，尽管CP中氨基的数量和/或结构会影响其吸附胆汁酸的能力。另一方面，二级胆汁酸牛磺脱氧胆酸盐比TCA和GCA更易被优先吸附。含有CS-OT的Alg-Ca无论凝胶基质的含水量如何，都以与CS-OT相似的方式摄取胆汁酸。由于所有成分都可作为食物，含有CS-OT的Alg-Ca可作为预防高脂血症的有用膳食剂，高脂血症是一种与生活方式相关的疾病。
