Montoro Paola, Piacente Sonia, Oleszek Wieslaw, Pizza Cosimo
Dipartimento di Scienze Farmaceutiche, Universitá di Salerno via Ponte don Melillo, 84084 Fisciano (SA), Italy.
J Mass Spectrom. 2004 Oct;39(10):1131-8. doi: 10.1002/jms.688.
Qualitative and quantitative analyses of phenolic compounds are of interest for both medicinal and food plants. In the present work, the phenolic fraction from Yucca schidigera, a plant bearing the GRAS (Generally Recognized as Safe) label approved by the US Food and Drug Administration, was studied. Crude extracts of Y. schidigera bark were investigated by liquid chromatography/UV spectrophotometry with diode-array detection, liquid chromatography/electrospray ionization mass spectrometry (LC/ESI-MS) and liquid chromatography/electrospray ionization tandem mass spectrometry (LC/ESI-MS/MS), in order to develop and optimize simple and rapid techniques to determine both stilbenes and yuccaols for the purposes of quality control of collected material. With optimal LC and MS conditions, stilbenes and yuccaols were quantified with all the proposed methods and the results were compared. Sensitivity was evaluated and the results indicated that MS/MS detection in the multiple reaction monitoring mode is easily applicable to this plant and allows the rapid and direct identification and quantification of these peculiar compounds in crude plant extracts.
酚类化合物的定性和定量分析对药用植物和食用植物都很重要。在本研究中，对丝兰（Yucca schidigera）的酚类成分进行了研究，该植物带有美国食品药品监督管理局批准的GRAS（一般认为安全）标签。采用液相色谱/紫外分光光度法结合二极管阵列检测、液相色谱/电喷雾电离质谱（LC/ESI-MS）和液相色谱/电喷雾电离串联质谱（LC/ESI-MS/MS）对丝兰树皮的粗提物进行了研究，目的是开发和优化简单快速的技术，用于测定所采集材料中的芪类化合物和丝兰醇，以进行质量控制。在最佳的液相色谱和质谱条件下，用所有提出的方法对芪类化合物和丝兰醇进行了定量，并对结果进行了比较。评估了灵敏度，结果表明，多反应监测模式下的串联质谱检测很容易应用于该植物，并能快速直接地鉴定和定量粗植物提取物中的这些特殊化合物。
