Montoro Paola, Skhirtladze Alexandre, Bassarello Carla, Perrone Angela, Kemertelidze Ether, Pizza Cosimo, Piacente Sonia
Dipartimento di Scienze Farmaceutiche, Università degli Studi di Salerno, via Ponte Don Melillo, 84084 Fisciano (SA), Italy.
J Pharm Biomed Anal. 2008 Aug 5;47(4-5):854-9. doi: 10.1016/j.jpba.2008.04.011. Epub 2008 May 23.
On the basis of the biological activities shown by yuccaols and gloriosaols from Yucca schidigera and Yucca gloriosa, the content of yuccaols and gloriosaols in two different parts of Y. gloriosa (roots and bark), was determined for each single compound, and compared with phenolic determination in Y. schidigera bark, concluding that Y. gloriosa bark and roots are rich sources of phenolic derivatives structurally related to resveratrol. LC/ESIMS (liquid chromatography coupled to electrospray mass spectrometry) qualitative and an LC/ESIMS/MS (liquid chromatography coupled to tandem electrospray mass spectrometry) quantitative studies of the phenolic fraction of Y. gloriosa were performed. LC/ESIMS/MS multiple reaction monitoring (MRM) method previously described for yuccaols in Y. schidigera was applied and optimised for separation and determination of gloriosaols and yuccaols in Y. gloriosa. Due to the sensitivity and the repeatability of the assay, we suggest this method as suitable for industrial quality control of raw materials and final products.
基于丝兰属植物（Yucca schidigera）和黄边丝兰（Yucca gloriosa）中的丝兰醇和黄边丝兰醇所显示的生物活性，针对黄边丝兰两个不同部位（根和树皮）中的丝兰醇和黄边丝兰醇含量，对每种单一化合物进行了测定，并与丝兰属植物树皮中的酚类物质测定结果进行了比较，得出结论：黄边丝兰的树皮和根是与白藜芦醇结构相关的酚类衍生物的丰富来源。对黄边丝兰的酚类组分进行了液相色谱/电喷雾质谱联用（LC/ESIMS）定性分析以及液相色谱/串联电喷雾质谱联用（LC/ESIMS/MS）定量研究。应用先前描述的用于丝兰属植物中丝兰醇的LC/ESIMS/MS多反应监测（MRM）方法，并对其进行优化，以分离和测定黄边丝兰中的黄边丝兰醇和丝兰醇。鉴于该测定方法的灵敏度和可重复性，我们建议该方法适用于原材料和最终产品的工业质量控制。
