Deng Jia-Hong, Fan Jian-Gang, Liang Chuan-Yu
Department of Otorhinolaryngology, West China Hospital of Sichuan University, Chengdu 610041, China.
Zhonghua Er Bi Yan Hou Ke Za Zhi. 2004 Jul;39(7):389-93.
To explore the electrophysiological properties of spiral ganglion neurons (SGN) isolated from neonatal mice.
Ion channel currents of acutely trypsin-dissociated spiral ganglion neurons was recorded and analyzed using whole-cell variation of the voltage clamp technique.
In the membrane of SGN, we recorded tetraethylammonium (TEA)-sensitive and 4-aminopyridine (4-AP)-sensitive outside delayed rectifier potassium currents, A-type potassium currents and tetrodotoxin-sensitive inside sodium currents. Ion channel activity had the character of voltage-dependence. Some cells, which did not exhibit sodium currents exhibited, delayed rectifier potassium currents.
The electrophysiological properties of acutely dissociated spiral ganglion neurons could be reference to the research of the mechanics of auditory propagation and the ion channel pharmacology.
探究从新生小鼠分离出的螺旋神经节神经元（SGN）的电生理特性。
采用电压钳技术的全细胞变体记录并分析经急性胰蛋白酶解离的螺旋神经节神经元的离子通道电流。
在SGN细胞膜上，我们记录到了对四乙铵（TEA）敏感和对4-氨基吡啶（4-AP）敏感的外向延迟整流钾电流、A型钾电流以及对河豚毒素敏感的内向钠电流。离子通道活性具有电压依赖性。一些未表现出钠电流的细胞表现出延迟整流钾电流。
急性解离的螺旋神经节神经元的电生理特性可为听觉传播机制及离子通道药理学的研究提供参考。
